PXD043640

PXD043640 is an original dataset announced via ProteomeXchange.

Title	Loss of Rnd3/RhoE induces entosis in human hepatocellular carcinoma
Description	Entosis is a process that leads to the formation of cell-in-cell structures commonly found in cancers. Here, we identified entosis in hepatocellular carcinoma and the loss of Rnd3 as an efficient inducer of this mechanism. We characterized the different stages and the molecular regulators of entosis induced after silencing of Rnd3. We demonstrated that this process is dependent on RhoA/ROCK pathway, but independent on E-cadherin. The proteomic analysis of entotic cells allowed us to identify LAMP-1 as a protein implicated not only in the degradation final stage of entosis, but also in the full mechanism. By analyzing entosis in human hepatocellular carcinoma samples, we found a relationship between the presence of entotic cells and the metastatic potential of tumors. Altogether, these data suggest the involvement of entosis in liver tumor progression and highlight a new perspective for the entosis analysis in medicine research as a novel therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:32:22.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-10 06:05:37	ID requested
⏵ 1	2024-01-26 10:32:23	announced

Basbous S, Dif L, Dantzer C, Di-Tommaso S, Dupuy JW, Bioulac-Sage P, Raymond AA, Desdouets C, Saltel F, Moreau V, Loss of RND3/RHOE controls entosis through LAMP1 expression in hepatocellular carcinoma. Cell Death Dis, 15(1):46(2024) [pubmed]

10.1038/s41419-024-06420-3;

submitter keyword: Rnd3, RhoA/ROCK, LAMP-1, hepatocellular carcinoma,Cell-in-cell, entosis, tumor progression.

Violaine Moreau
contact affiliation	Univ. Bordeaux, INSERM, BRIC, U1312, Bordeaux, France
contact email	violaine.moreau@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043640

PRIDE project URI

• PRIDE
  1. PXD043640
     1. Label: PRIDE project
     2. Name: Loss of Rnd3/RhoE induces entosis in human hepatocellular carcinoma