PXD043623

PXD043623 is an original dataset announced via ProteomeXchange.

Title	An oligosaccharyltransferase from Leishmania donovani increases the N-glycan occupancy on plant-produced IgG1
Description	The monoclonal antibodies rituximab and cetuximab were transiently expressed in leaves of Nicotiana benthamiana plants and purified by affinity chromatography using protein A. Purified antibodies were analysed by intact MS. Purified antibodies were also subjected to trypsin digestion followed by LC-ESI-MS analysis of the glycopeptide carrying the N-glycsylation site Asn297 using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:50:26.589.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Strasser
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	complex glycosylation
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-09 03:48:49	ID requested
1	2023-07-31 06:46:19	announced
⏵ 2	2023-11-14 06:50:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: recombinant protein, antibody, oligosaccharyltransferase,N-glycosylation

Richard Strasser
contact affiliation	Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria
contact email	richard.strasser@boku.ac.at
lab head
Richard Strasser
contact affiliation	University of Natural Resources and Life Sciences Vienna
contact email	richard.strasser@boku.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD043623

PRIDE project URI

• PRIDE
  1. PXD043623
     1. Label: PRIDE project
     2. Name: An oligosaccharyltransferase from Leishmania donovani increases the N-glycan occupancy on plant-produced IgG1