PXD043615

PXD043615 is an original dataset announced via ProteomeXchange.

Title	Venomics of the Milos viper (Macrovipera schweizeri)
Description	Snakebite is a neglected tropical disease and a globally important driver of death and morbidity. Vipers of the genus Macrovipera (Viperidae: Viperinae) are among the snakes of higher medical importance in the Old World. Despite the medical relevance of Macrovipera venoms, the knowledge regarding them is heterogeneously distributed with virtually all works conducted so far focusing on subspecies of Macrovipera lebetinus, while other species within the genus are largely overlooked. Here we present the first proteomic evaluation of the venom from the Greek endemic Milos viper (Macrovipera schweizeri). In line with clinical symptoms typically elicited by Macrovipera envenomations, Milos viper venom primarily comprises coagulotoxic and cytotoxic protein families, such as metalloproteinases (svMP) and serine proteases (svSP). We conducted comparative bioactivity assays on venoms from M. schweizeri and the M. lebetinus subspecies M. lebetinus cernovi, M. lebetinus obtusa, and M. lebetinus turanica, and showed that they all exhibit similarities in levels of cytotoxicity proteolytic activity, and inhibition of prokaryotic growth. Lastly, we compared 1D-SDS-PAGE and RP-HPLC Macrovipera spp. venom profiles, as well as our proteomic data with previously published Macrovipera venom proteomes. The analyses performed reveal that a general venom profile seems to be conserved across blunt-nosed vipers, and that, similarly to M. lebetinus venom, M. schweizeri venom is able to cause significant tissue damage. The present work represents an important starting point to the development of comparative studies across the full taxonomic range of Macrovipera spp., and can potentially help optimize the treatment of envenomations caused by M. schweizeri.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:11:35.871.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043615
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Günter Lochnit
SpeciesList	scientific name: Macrovipera schweizeri; NCBI TaxID: 110205;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-08 00:22:26	ID requested
1	2023-10-24 13:32:41	announced
⏵ 2	2023-11-14 09:11:37	announced	2023-11-14: Updated project metadata.

10.6019/PXD043615;

Schulte L, Damm M, Avella I, Uhrig L, Erkoc P, Schiffmann S, F, ü, rst R, Timm T, Lochnit G, Vilcinskas A, L, ü, ddecke T, ) unveils patterns of venom composition and exochemistry across blunt-nosed viper venoms. Front Mol Biosci, 10():1254058(2023) [pubmed]

submitter keyword: proteomics,Blunt-nosed vipers, venom, snakebite, bioactivity

Guenter Lochnit
contact affiliation	Protein Analytics, INstitute of Biochemistry, Faculty of medicine, University Giessen
contact email	guenter.lochnit@biochemie.med.uni-giessen.de
lab head
Günter Lochnit
contact affiliation	Protein Analytics, Institute of Biiochemistry, Faculty of Medicine, University Giessen, Friedrichstrasse 24, 35392 Giessen
contact email	guenter.lochnit@biochemie.med.uni-giessen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043615
     1. Label: PRIDE project
     2. Name: Venomics of the Milos viper (Macrovipera schweizeri)