PXD043614
PXD043614 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LD4172 100nM and LD4172NC 100nM treated MDA_MB_231 cell line global proteomics |
| Description | 1 million MDAMB231 cells were seeded in 6-well plates. On the following day, MBAMB231 cells were treated in triplicates with DMSO, LD4172 (100 nM) or LD4172-NC (100 nM) for 6 hours. Cells were washed with ice-cold PBS three times. The cell pellets were lysed, reduced, alkylated, and digested by EasyPep MS Sample Prep Kits (ThermoFisher: A45733). The same amount of peptide from each condition was labeled with tandem mass tag (TMT) reagent (ThermoFisher: 90113). The 10-plex TMT reagents were incubated with each peptide sample for 1 hour at room temperature. The labeled peptide samples were quenched by adding 50 uL of 5% hydroxylamine, 20% formic acid solution and mixed. Mixed samples were desalted and offline fractionated into 24 fractions as previously described. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-08-28 |
| AnnouncementXML | Submission_2024-08-28_17:43:35.081.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jin Wang |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-07 22:07:51 | ID requested | |
| ⏵ 1 | 2024-08-28 17:43:35 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: RIPK1 PROTAC, MDA-MB-231 |
Contact List
| Jin Wang | |
|---|---|
| contact affiliation | Baylor College of Medicine, Houston TX |
| contact email | wangj@bcm.edu |
| lab head | |
| Jin Wang | |
| contact affiliation | Baylor College of Medicine |
| contact email | wangj@bcm.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092377/ |
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