PXD043605

PXD043605 is an original dataset announced via ProteomeXchange.

Title	3D bioprinting directly affects proteomic signature and myogenic maturation in muscle pericytes-derived human myo-substitute
Description	Skeletal muscle tissue engineering (SMTE) has recently emerged to address major clinical challenges such as volumetric muscle loss. Here, we report a rotary wet-spinning (RoWS) biofabrication technique for producing human myo-substitutes with biomimetic architectures and functions. Here, we demonstrate how the proposed technique may be used to establish a well-tailored, anisotropic microenvironment that promotes exceptional myogenic differentiation of human skeletal muscle-derived pericytes (hPeri). Using high-resolution mass spectrometry-based proteomics with the integration of literature-derived signaling networks, we uncovered that i) 3D biomimetic matrix environment (PEG-Fibrinogen) confers a lower mitogenicity microenvironment compared to standard 2D cultures, favoring the formation of contractile-competent bundles of pericytes-derived myotubes in an anchoring-independent 3D state, and ii) the bioprinting method promotes an upregulation of muscle matrix structural protein besides increasing contractile machinery proteins with respect to 3D bulk cultures. Finally, in vivo investigations demonstrate that the 3D bioprinted myo-substitute is fully compatible with the host ablated muscular tissue, exhibiting myo-substitute engraftment and muscle regeneration in a mouse VML model. Overall, the results show that 3D bioprinting has a superior capability for controlling the myogenic differentiation process on a macroscale and, with future refining, may have the potential to be translated into clinical practice.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-20_08:54:07.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessio Reggio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-07 05:46:48	ID requested
⏵ 1	2025-10-20 08:54:08	announced

10.1002/AGT2.727;

submitter keyword: myogenic maturation, myosubstitute, bioprinting, perycites

Cesare Gargioli
contact affiliation	Department of Biology, Rome University of Rome Tor Vergata, Rome, IT.
contact email	cesare.gargioli@uniroma2.it
lab head
Alessio Reggio
contact affiliation	Saint Camillus International University of Healt Sciences
contact email	alessio.reggio@uniroma2.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD043605

PRIDE project URI

• PRIDE
  1. PXD043605
     1. Label: PRIDE project
     2. Name: 3D bioprinting directly affects proteomic signature and myogenic maturation in muscle pericytes-derived human myo-substitute