PXD043603

PXD043603 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling proteins differences between TRIM25 and G3BP1 interactions or not, and Proximity labeling proteins differences between G3BP1 and TRIM25 in the presence or absence of poly(I:C) stimulation
Description	This project investigates G3BP1 proximity proteins by constructing a G3BP1-BirA* fusion protein using G3BP1 as a decoy protein and BirA* as a biotin ligase. The changes in G3BP1 proximity proteins were studied in the presence or absence of poly(I:C) stimulation conditions, using poly(I:C) stimulation as a variable. This project investigated the TRIM25 proximity protein by constructing a TRIM25-BirA* fusion protein with TRIM25 as the decoy protein and BirA* as the biotin ligase. The changes of TRIM25 proximity proteins under the conditions of TRIM25 interacting with or without G3BP1 were studied by performing PTFG sequence deletion on TRIM25; the changes in TRIM25 proximity proteins were studied in the presence or absence of poly(I:C) stimulation conditions, using poly(I:C) stimulation as a variable.
HostingRepository	iProX
AnnounceDate	2023-07-07
AnnouncementXML	Submission_2025-05-21_18:59:12.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zehua Shang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-07 03:37:51	ID requested
1	2023-07-07 03:37:58	announced
⏵ 2	2025-05-21 18:59:13	announced	2025-05-22: Add Publication.

Shang Z, Zhang S, Wang J, Zhou L, Zhang X, Billadeau DD, Yang P, Zhang L, Zhou F, Bai P, Jia D, TRIM25 predominately associates with anti-viral stress granules. Nat Commun, 15(1):4127(2024) [pubmed]

submitter keyword: TRIM25, G3BP1, BioID, BirA*, poly(I:C)

Da Jia
contact affiliation	Sichuan University
contact email	Jiada_SCU@163.com
lab head
Zehua Shang
contact affiliation	Sichuan University
contact email	shangzehua990214@163.com
dataset submitter

iProX dataset URI