PXD043583

PXD043583 is an original dataset announced via ProteomeXchange.

Title	Cell Lineage-Guided Microanalytical Mass Spectrometry Reveals Increased Energy Metabolism and Reactive Oxygen Species in the Vertebrate Organizer
Description	The vertebrate body plan is established through inductive signaling during gastrulation via a signaling center, which is called the Spemann-Mangold organizer (SMO) in the developmental model Xenopus laevis, the South African Clawed Frog. Here, we performed a multiplexed quantitative proteomic screen of the SMO in contrast with the rest of the embryo (RE). Wild-type embryos were cultured to the 32-cell stage, where precursor cells of the SMO were injected with a fluorescent lineage tracing dye. These experimental embryos were cultured to stage 10 (beginning of gastrulation), whence the fluorescently labelled cells were dissected. The proteome of the SMO and the RE were quantified using multiplexing quantification. The tissue samples were processed using a bottom-up proteomic workflow and analyzed using LC-MS3. The resulting data revealed an upregulation of oxidative phosphorylation (OXPHOS) in the SMO tissues.
HostingRepository	PRIDE
AnnounceDate	2024-01-10
AnnouncementXML	Submission_2024-01-10_15:43:47.724.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043583
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-06 09:34:55	ID requested
⏵ 1	2024-01-10 15:43:48	announced

10.1073/PNAS.2311625121;

10.6019/PXD043583;

submitter keyword: metabolism, proteomics, development, Spemann-Mangold organizer,Xenopus laevis, organizer

Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043583

PRIDE project URI

• PRIDE
  1. PXD043583
     1. Label: PRIDE project
     2. Name: Cell Lineage-Guided Microanalytical Mass Spectrometry Reveals Increased Energy Metabolism and Reactive Oxygen Species in the Vertebrate Organizer