PXD043541

PXD043541 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanisms underlying intraspecific variation in snake venom
Description	Elucidating the molecular mechanisms underlying snake venom variability provides important clues for understanding how the biological functions of this powerful toxic arsenal evolve. We analyzed in detail individual transcripts and venom protein isoforms produced by five specimens of a venomous snake (Bothrops atrox) from two nearby but genetically distinct populations from the Brazilian Amazon rainforest which show functional similarities in venom properties. Individual variation was observed among the venoms of these specimens, but the overall abundance of each general toxin family was conserved both in transcript and in venom protein levels. However, when expression of independent paralogues was analyzed, remarkable differences were observed within and among each toxin group, both between individuals and between populations. Transcripts for functionally essential venom proteins (“core function” proteins) were highly expressed in all specimens and showed similar transcription/translation rates. In contrast, other paralogues (“adaptive” proteins) showed lower expression levels and the toxins they coded for varied among different individuals. These results provide support for the inferences that (a) expression and translational differences play a greater role in defining adaptive variation in venom phenotypes than does sequence variation in protein coding genes and (b) convergent adaptive venom phenotypes can be generated through different molecular mechanisms. Significance: Analysis of individual transcripts and venom protein isoforms produced by specimens of a venomous snake (Bothrops atrox), from the Brazilian Amazon rainforest, revealed that transcriptional and translational mechanisms contribute to venom phenotypic variation. Our finding of evidence for high expression of toxin proteins with conserved function supports the hypothesis that the venom phenotype consists of two kinds of proteins: conserved “core function” proteins that provide essential functional activities with broader relevance and less conserved “adaptive” proteins that vary in expression and may permit customization of protein function. These observations allowed us to suggest that genetic mechanisms controlling venom variability are not restricted to selection of gene copies or mutations in structural genes but also to selection of the mechanisms controlling gene expression, contributing to the plasticity of this important phenotype for venomous snakes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:59.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Hemmi Valente
SpeciesList	scientific name: Bothrops atrox; NCBI TaxID: 8725;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-04 13:26:46	ID requested
1	2023-07-04 13:40:35	announced
⏵ 2	2023-11-14 09:02:07	announced	2023-11-14: Updated project metadata.

Amazonas DR, Portes-Junior JA, Nishiyama-Jr MY, Nicolau CA, Chalkidis HM, Mour, ã, o RHV, Grazziotin FG, Rokyta DR, Gibbs HL, Valente RH, Junqueira-de-Azevedo ILM, Moura-da-Silva AM, Molecular mechanisms underlying intraspecific variation in snake venom. J Proteomics, 181():60-72(2018) [pubmed]

submitter keyword: Plasticity,Snake venom, Toxin function, Proteome, Evolution, Transcriptome

Richard Hemmi Valente
contact affiliation	Laboratory of Toxinology FIOCRUZ - Rio de Janeiro - Brazil
contact email	richardhemmi@gmail.com
lab head
Richard Hemmi Valente
contact affiliation	Fundação Oswaldo Cruz
contact email	richardhemmi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD043541

PRIDE project URI

• PRIDE
  1. PXD043541
     1. Label: PRIDE project
     2. Name: Molecular mechanisms underlying intraspecific variation in snake venom