⮝ Full datasets listing

PXD043524

PXD043524 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial fuels ATP in the nucleus to prevent cell cycle arrest during mechanical confinement
DescriptionAbstract The physical microenvironment regulates cell behaviour during tissue development and disease. Whether mechanical stress and physical space constraints reshape the subcellular localization of organelles and proteins resulting in functional adaptations is, however, unknown. Here, we performed proteomic analysis to understand how proteins reorganise between the nucleus and the cytoplasm when cancer cells are acutely confined. We discovered that cellular confinement induces a strong enrichment of mitochondrial proteins within the nuclear compartment. High-resolution microscopy confirmed that upon confinement mitochondria, in an actin-dependent manner, are trapped within an ER-based net that indents the nucleus. At the functional level, the close proximity of mitochondria and the nucleus results in a nuclear ATP surge, which is reverted by inhibiting ATP mitochondrial export or actin polymerisation. Pharmacological prevention of this confinement-induced nuclear ATP surge impedes cell proliferation through cell cycle defects. Taken together, our data indicates that the physical association between the nucleus and mitochondria is a functional requirement for cell cycle completion after acute mechanical stress.
HostingRepositoryPRIDE
AnnounceDate2025-06-12
AnnouncementXMLSubmission_2025-06-12_07:14:14.572.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSavvas Kourtis
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-04 02:34:58ID requested
12025-06-12 07:14:14announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Confinement, nuclear ATP, mitochondria, cell cycle, nucleus, DNA damage
Contact List
Sara Sdelci
contact affiliationCenter for Genomic Research (CRG), Barcelona
contact emailsara.sdelci@crg.eu
lab head
Savvas Kourtis
contact affiliationCRG
contact emailkourtissavvas@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD043524
PRIDE project URI
Repository Record List
[ + ]