PXD043524 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mitochondrial fuels ATP in the nucleus to prevent cell cycle arrest during mechanical confinement |
Description | Abstract The physical microenvironment regulates cell behaviour during tissue development and disease. Whether mechanical stress and physical space constraints reshape the subcellular localization of organelles and proteins resulting in functional adaptations is, however, unknown. Here, we performed proteomic analysis to understand how proteins reorganise between the nucleus and the cytoplasm when cancer cells are acutely confined. We discovered that cellular confinement induces a strong enrichment of mitochondrial proteins within the nuclear compartment. High-resolution microscopy confirmed that upon confinement mitochondria, in an actin-dependent manner, are trapped within an ER-based net that indents the nucleus. At the functional level, the close proximity of mitochondria and the nucleus results in a nuclear ATP surge, which is reverted by inhibiting ATP mitochondrial export or actin polymerisation. Pharmacological prevention of this confinement-induced nuclear ATP surge impedes cell proliferation through cell cycle defects. Taken together, our data indicates that the physical association between the nucleus and mitochondria is a functional requirement for cell cycle completion after acute mechanical stress. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-12 |
AnnouncementXML | Submission_2025-06-12_07:14:14.572.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Savvas Kourtis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-04 02:34:58 | ID requested | |
⏵ 1 | 2025-06-12 07:14:14 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Confinement, nuclear ATP, mitochondria, cell cycle, nucleus, DNA damage |
Contact List
Sara Sdelci |
contact affiliation | Center for Genomic Research (CRG), Barcelona |
contact email | sara.sdelci@crg.eu |
lab head | |
Savvas Kourtis |
contact affiliation | CRG |
contact email | kourtissavvas@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043524
- Label: PRIDE project
- Name: Mitochondrial fuels ATP in the nucleus to prevent cell cycle arrest during mechanical confinement