PXD043520

PXD043520 is an original dataset announced via ProteomeXchange.

Title	Physiological implications of dietary inclusion of the algae Laminaria digitata on the weaned piglet: a combined proteomics and metabolomics approach
Description	The brown seaweed Laminaria digitata is a novel feedstuff for weaned piglets. It can help prevent dysbiosis in addition to improve overall health and performance. However, it has a recalcitrant cell wall that is not easily digested by the piglet digestive system. Alginate lyase has promising effects for the supplementation of in vivo diets in order to address this issue. The objective of this study is to evaluate the effect of 10% dietary Laminaria digitata inclusion and alginate lyase supplementation on the hepatic proteome and metabolome of weaned piglets in a physiological study. The diets caused incipient differences on the metabolome of piglets, with the proteome having the most significant changes. Feeding seaweed provided a source of n-3 PUFA that accumulated in the liver, signalling for increased fatty acid oxidation (FABP, ACADSB, ALDH1B1). This contributed at least in part to reduce oxidative stability of the tissue, demanding the higher abundance of GST to maintain it. Reactive oxygen species possibly damaged proteins, which caused hepatocytes to increase proteasome activity (LAPTM4B, PSMD4), recycling their amino acids. Providing alginate lyase to the diet increased the number of differentially abundant proteins, including GBE1 and LDHC that contributed to the maintenance of circulating glucose through mobilization of glycogen stores and branched chain amino acids. Enzymatic supplementation enhanced the baseline effects of feeding seaweed alone.
HostingRepository	PRIDE
AnnounceDate	2024-01-05
AnnouncementXML	Submission_2024-01-05_00:16:17.313.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043520
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	L-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-04 00:29:50	ID requested
⏵ 1	2024-01-05 00:16:17	announced

10.6019/PXD043520;

10.1016/J.JPROT.2023.105063;

submitter keyword: proteomics, piglet, metabolomics,Laminaria digitata

Celine LECLERCQ
contact affiliation	LIST-ERIN-BEAP Bascharage1 Luxembourg
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-BEAP-HAU platform
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043520
     1. Label: PRIDE project
     2. Name: Physiological implications of dietary inclusion of the algae Laminaria digitata on the weaned piglet: a combined proteomics and metabolomics approach