⮝ Full datasets listing

PXD043520

PXD043520 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhysiological implications of dietary inclusion of the algae Laminaria digitata on the weaned piglet: a combined proteomics and metabolomics approach
DescriptionThe brown seaweed Laminaria digitata is a novel feedstuff for weaned piglets. It can help prevent dysbiosis in addition to improve overall health and performance. However, it has a recalcitrant cell wall that is not easily digested by the piglet digestive system. Alginate lyase has promising effects for the supplementation of in vivo diets in order to address this issue. The objective of this study is to evaluate the effect of 10% dietary Laminaria digitata inclusion and alginate lyase supplementation on the hepatic proteome and metabolome of weaned piglets in a physiological study. The diets caused incipient differences on the metabolome of piglets, with the proteome having the most significant changes. Feeding seaweed provided a source of n-3 PUFA that accumulated in the liver, signalling for increased fatty acid oxidation (FABP, ACADSB, ALDH1B1). This contributed at least in part to reduce oxidative stability of the tissue, demanding the higher abundance of GST to maintain it. Reactive oxygen species possibly damaged proteins, which caused hepatocytes to increase proteasome activity (LAPTM4B, PSMD4), recycling their amino acids. Providing alginate lyase to the diet increased the number of differentially abundant proteins, including GBE1 and LDHC that contributed to the maintenance of circulating glucose through mobilization of glycogen stores and branched chain amino acids. Enzymatic supplementation enhanced the baseline effects of feeding seaweed alone.
HostingRepositoryPRIDE
AnnounceDate2024-01-05
AnnouncementXMLSubmission_2024-01-05_00:16:17.313.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD043520
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCéline LECLERCQ
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListL-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-04 00:29:50ID requested
12024-01-05 00:16:17announced
Publication List
10.6019/PXD043520;
10.1016/J.JPROT.2023.105063;
Keyword List
submitter keyword: proteomics, piglet, metabolomics,Laminaria digitata
Contact List
Celine LECLERCQ
contact affiliationLIST-ERIN-BEAP Bascharage1 Luxembourg
contact emailceline.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliationLIST-ERIN-ERTP-BEAP-HAU platform
contact emailceline.leclercq@list.lu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043520
PRIDE project URI
Repository Record List
[ + ]