PXD043492

PXD043492 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Deubiquitinating enzymes in human macrophages after Francisella tularensis infection
Description	Ubiquitination/deubiquitination belong to the most important regulatory mechanisms carried out through the attachment/removal of the ubiquitin molecule, respectively. The process is necessary not only to mark molecules for degradation, but also for example to the activation of signaling pathways leading to pro-inflammatory host response. Many intracellular pathogens, such as Francisella tularensis, have evolved mechanisms of blocking such host immune responses to escape degradation. Here, we describe that F. tularensis interferes with the host's ubiquitination system. We showed increased total activity of deubiquitinating enzymes (DUBs) in human macrophages after infection and confirmed the reduced enzymatic activities of two specific DUBs: USP10 and UCH-L5, and the increased activity of USP25. We further revealed the enrichment of these three enzymes in exosomes derived from F. tularensis-infected cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:12:33.194.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043492
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pavel Rehulka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-03 06:36:14	ID requested
1	2023-10-24 16:18:37	announced
⏵ 2	2023-11-14 09:12:34	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD043492;
Vozandychova V, Rehulka P, Hercik K, Spidlova P, Pavlik P, Hanus J, Hadravova R, Stulik J, infection. Front Immunol, 14():1252827(2023) [pubmed]

10.6019/PXD043492;

Vozandychova V, Rehulka P, Hercik K, Spidlova P, Pavlik P, Hanus J, Hadravova R, Stulik J, infection. Front Immunol, 14():1252827(2023) [pubmed]

Keyword List

submitter keyword: Human, macrophage, Francisella tularensis, LC-MS, host-pathogen

submitter keyword: Human, macrophage, Francisella tularensis, LC-MS, host-pathogen

Contact List

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Pavel Rehulka
contact affiliation	Department of Molecular Pathology and Biology FMHS UD, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic
contact email	pavel.rehulka@unob.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043492
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043492

PRIDE project URI

Repository Record List

[ + ]