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PXD043474

PXD043474 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUsing SWATH-MS to Identify New Molecular Biomarkers in Gingival Crevicular Fluid for Detecting Periodontitis and its Response to Treatment
DescriptionOver the last years, proteomic techniques have been demonstrated to be valuable tools for analysing the periodontal proteome in gingival crevicular fluid (GCF) samples contributing to identifying extensive and comprehensive protein profiles associated with periodontitis. However, the periodontal proteome has not yet been studied using sequential window acquisition of all theoretical mass spectra (SWATH-MS). Consequently, the objective of the present longitudinal research was to investigate the periodontal proteome in GCF in three clinical conditions (periodontal health, untreated periodontitis, and treated periodontitis), applying SWATH-MS to 1) analyse the periodontal proteome structure in the three clinical groups, 2) compare the differential protein expression between the three clinical groups, and 3) evaluate the diagnostic accuracy of the proteins quantified to identify new biomarkers with a high capacity to discriminate the three clinical conditions. A total of 84 subjects, 44 periodontally healthy and 40 with generalised untreated periodontitis in stages III-IV, were included, and GCF samples were collected. The diagnosis of periodontal status was conducted by two experienced dentists using clinical and radiographic criteria. The definition of periodontal status was established following the Classification of Periodontal Diseases and Conditions published in 2018. In the untreated periodontitis group, 38 underwent conventional non-surgical treatment and were evaluated clinically after two months (treated periodontitis group). In the revaluation, 25 individuals showed significant clinical improvement in probing pocket depth (PPD) at the selected sites, and GCF samples were collected from them.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_04:02:22.083.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusana Bravo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-03 02:16:15ID requested
12024-10-17 04:02:22announced
Publication List
Blanco-Pintos T, Regueira-Iglesias A, Kuz I, S, á, nchez-Barco A, Seijas-Otero N, Chantada-V, á, zquez MDP, Balsa-Castro C, Tom, á, s I, Impact of smoking habit on the subgingival proteome in patients with periodontitis. J Periodontol, ():(2024) [pubmed]
10.1002/jper.24-0062;
Keyword List
submitter keyword: gingival crevicular fluid, mass spectrometry,Diagnostic accuracy, molecular biomarkers, SWATH-MS, periodontitis
Contact List
Susana Belen Bravo Lopez
contact affiliationInstituto de Investigaciones sanitarias de Santiago de Compostela
contact emailsbbravo@gmail.com
lab head
Susana Bravo
contact affiliationFIDIS
contact emailsbbravo@gmail.com
dataset submitter
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