PXD043456

PXD043456 is an original dataset announced via ProteomeXchange.

Title	Data in support of Comparative Proteomics Analysis in Different Stages of Urothelial Bladder Cancer for Identification of Potential Biomarkers
Description	Non-muscle-invasive bladder cancer (NMIBC) has a high recurrence rate and muscle-invasive bladder cancer (MIBC) has unfavorable outcomes in urothelial bladder cancer (UBC) patients. Complex UBC-related protein biomarkers for outcome prediction may provide a more efficient management approach with an improved clinical outcome. The aim of this study is to recognize tumor-associated proteins, which are differentially expressed in different stages of UBC patients compared non-cancerous tissues. Methods: The proteome of tissue samples of 42 UBC patients (NMIBC n=25 and MIBC n=17) was subjected to two-dimensional electrophoresis (2-DE) combined with Liquid chromatography–mass spectrometry (LC–MS) system to identify differentially expressed proteins. The intensity of protein spots was quantified and compared with SameSpots software. Functional, pathway, and interaction analyses of identified proteins were performed using geneontology (GO), PANTHER, Reactome, Gene MANIA, and STRING databases. Results: Twelve proteins identified by LC-MS showed differential expression (over 1.5-fold, p<0.05), including 9 up-regulated in NMIBC and 3 up-regulated in MIBC patients. Proteins involved in the detoxification of reactive oxygen species and cellular responses to oxidative stress showed the most significant changes in UBC patients. Additionally, the most potential functions related to these detected proteins were associated with the peroxidase, oxidoreductase, and antioxidant activity.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:45:48.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Stensballe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-02 22:03:27	ID requested
⏵ 1	2025-05-06 09:45:49	announced

10.1007/s12672-025-02167-1;

Tabaei S, Zareinejad M, Haghshenas MR, Shakhssalim N, Gilany K, Stensballe A, Ghaderi A, Investigation of new autoantibodies in urothelial bladder cancer for biomarker discovery using immunoproteomics. Discov Oncol, 16(1):436(2025) [pubmed]

submitter keyword: Non-muscle-invasive bladder cancer, muscle-invasive bladder cancer

Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
lab head
Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043456
     1. Label: PRIDE project
     2. Name: Data in support of Comparative Proteomics Analysis in Different Stages of Urothelial Bladder Cancer for Identification of Potential Biomarkers