PXD043452

PXD043452 is an original dataset announced via ProteomeXchange.

Title	PARP14 is a PARP with both ADP-ribosyl transferase 1 and hydrolase activities
Description	PARP14 is a mono-ADP-ribosyl transferase involved in the control of immunity, transcription and DNA replication stress management. However, little is known about the ADP-ribosylation activity of PARP14, including its substrate specificity or how PARP14-dependent ADP-ribosylation is reversed. Here we show that PARP14 is dual function enzyme with both ADP-ribosyl transferase and hydrolase activity acting on both protein and nucleic acid substrates. In particular, we show that the PARP14 macrodomain 1 is an active ADP-ribosyl hydrolase. We also demonstrate hydrolytic activity for the first macrodomain of PARP9. We reveal that expression of a PARP14 mutant with the inactivated macrodomain 1 results in a dramatic increase in mono(ADP-ribosyl)ation of proteins in human cells, including PARP14 itself and antiviral PARP13. Moreover, we demonstrate that the closely related hydrolytically active macrodomain of SARS2 Nsp3, Mac1, efficiently reverses PARP14 ADP-ribosylation in vitro and in cells, supporting the evolution of viral macrodomains to counteract PARP14-mediated antiviral response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:14.154.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Elsborg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-02 16:39:05	ID requested
1	2023-08-14 01:41:08	announced
2	2023-11-14 06:50:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:00:14	announced	2024-10-22: Updated project metadata.

10.1126/SCIADV.ADI2687;

submitter keyword: innate immunity, DNA-repair, PARP13, SARS-CoV, Af1521-interactome, Ubiquitin signaling, ADPr hydrolase, PARP9, ADP-ribosylation,PARP14, mono-ADPr, Antiviral response

Michael Lund
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
Jonas Elsborg
contact affiliation	NNF Center for Protein Research
contact email	jonas.elsborg@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043452

PRIDE project URI

• PRIDE
  1. PXD043452
     1. Label: PRIDE project
     2. Name: PARP14 is a PARP with both ADP-ribosyl transferase 1 and hydrolase activities