PXD043450

PXD043450 is an original dataset announced via ProteomeXchange.

Title	Time of day effects on post-exercise phosphoproteomic profiling in mouse hippocampus
Description	At both the rest phase (ZT3) and the active phase (ZT15) for mice, the acute exercise mouse model and the sham-exercise group were established. Left hippocampi from every two mice were mixed as one sample and three replicates were adopted in each group. Then the hippocampal phosphoproteome and proteome were screened by affinity enrichment and LC-MS/MS. Aspects of differentially phosphorylated sites and proteins between the rest and active phase were compared, including functional enrichment analysis, PPI network analysis, motif analysis and kinase prediction, etc. as a result, exercise significantly changed 932 and 828 phosphosites at the early rest phase and the early active phase, respectively. There were only 49 overlapped differentially regulated sites on 44 proteins. Functional enrichment analysis displayed that the breadth of signaling pathways modulated by exercise at ZT3 was far greater than that at ZT15. The phosphorylation status of differentially phosphorylated proteins enriched in multiple pathways was vastly different after the timed exercise, so were the predicted upstream kinases. Up to 29% differentially phosphorylated proteins were related to the structure or function of synapses, and both exercise at ZT3 and ZT15 might significantly affect the pathway of the glutamate synapse-calcium signaling pathway-long-term potential in hippocampus.
HostingRepository	PRIDE
AnnounceDate	2026-03-20
AnnouncementXML	Submission_2026-03-20_01:15:16.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shan Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-02 09:04:32	ID requested
⏵ 1	2026-03-20 01:15:17	announced

Dataset with its publication pending

submitter keyword: Exercise

Circadian rhythm

Hippocampus

Synapse

Phosphorylation

Ping Qian
contact affiliation	Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, China
contact email	qianpingerke@163.com
lab head
Shan Wang
contact affiliation	Capital Institute of Pediatrics, Beijing, China
contact email	wsaquarius@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD043450

PRIDE project URI

• PRIDE
  1. PXD043450
     1. Label: PRIDE project
     2. Name: Time of day effects on post-exercise phosphoproteomic profiling in mouse hippocampus