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PXD043431

PXD043431 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics of sugarcane smut fungus - Sporisorium scitamineum unravels dynamic proteomic alterations during the dimorphic transition
DescriptionDimorphic fungi have the ability to change morphology during their lifecycle, a crucial feature for the establishment of infection and fungal growth and development in planta. Life cycle of the dimorphic sugarcane smut fungi, Sporisorium scitamineum, involves recognition and mating of compatible saprophytic yeast-like haploid sporidia (MAT-1 and MAT-2) that upon fusion, develop into infective dikaryotic mycelia. Although the dimorphic transition is intrinsically linked with the pathogenicity and virulence of S. scitamineum, it has never been studied using a proteomics approach. In the present study, an iTRAQ-based comparative proteomic analysis of three distinct stages covering the dimorphic transition period - haploid sporidial stage (MAT-1 and MAT-2) to the transition phase (24 hours post co-culturing (hpc)) and dikaryotic mycelial stage (48 hpc) was carried out. Functional categorization showed that the most altered biological processes were energy production, primary metabolism especially carbohydrate, amino acid, fatty acid, followed by translation, post-translation and protein turnover. The identified proteins could be grouped into 8 distinct clusters with different trends in abundance. Enrichment analysis of the clusters showed that biological processes related to energy production through oxidative phosphorylation, citrate cycle, and β-oxidation, transcription, translation and redox homeostasis were specifically altered. In addition, an overall downregulation of carbohydrate metabolism and reprogrammed amino acid metabolism were observed. Several differentially abundant proteins (DAPs), especially in the dikaryotic mycelial stage were predicted as effectors. Taken together, key molecular mechanisms underpinning the dimorphic transition in S. scitamineum at the proteome level were highlighted. A catalogue of stage-specific and dimorphic transition-associated -proteins and potential effectors identified herein are potential candidates for defective mutant screening to elucidate their functional role in the dimorphic transition and pathogenicity in S. scitamineum.
HostingRepositoryPRIDE
AnnounceDate2024-06-27
AnnouncementXMLSubmission_2024-06-27_05:02:52.592.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGiorgio Arrigoni
SpeciesList scientific name: Sporisorium scitamineum; NCBI TaxID: 49012;
ModificationListmonohydroxylated residue; iodoacetic acid derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-30 06:10:52ID requested
12024-06-27 05:02:53announced
Publication List
10.1016/j.jprot.2024.105230;
Kumaravel N, Ebinezer LB, Ashwin NMR, Franchin C, Battisti I, Carletti P, Ramesh Sundar A, Masi A, Malathi P, Viswanathan R, Arrigoni G, Comparative proteomics of sugarcane smut fungus - Sporisorium scitamineum unravels dynamic proteomic alterations during the dimorphic transition. J Proteomics, 304():105230(2024) [pubmed]
Keyword List
submitter keyword: Fungi
Dimorphism
Dikaryotic
Virulence
Pathogenesis
iTRAQ
Contact List
Giorgio Arrigoni
contact affiliationDepartment of Biomedical Sciences, University of Padova, Italy
contact emailgiorgio.arrigoni@unipd.it
lab head
Giorgio Arrigoni
contact affiliationUniversity of Padova
contact emailgiorgio.arrigoni74@gmail.com
dataset submitter
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