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PXD043425

PXD043425 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTemporal phosphoproteomics reveals circuitry of phased propagation in insulin signaling
DescriptionInsulin is a pleiotropic hormone that elicits its metabolic and mitogenic actions through numerous rapid and reversible protein phosphorylations. The temporal regulation of insulin’s intracellular signaling cascade is highly complex and insufficiently understood. We conducted a time-resolved analysis of the global insulin-regulated phosphoproteome of differentiated human primary myotubes derived from satellite cells of healthy donors using high-resolution mass spectrometry. Identification and tracking of ~13,000 phosphosites over time revealed a highly complex and coordinated network of transient phosphorylation and dephosphorylation events that can be allocated to time-phased regulation of distinct and non-overlapping subcellular pathways. Advanced network analysis combining protein-protein-interaction (PPI) resources and investigation of donor variability in phosphosite occupancy over time identifies novel putative candidates in non-canonical insulin signaling and key regulatory nodes that are likely essential for signal propagation. Lastly we found that insulin-regulated phosphorylation of the pre-catalytic spliceosome complex is associated with acute alternative splicing events in the transcriptome of human skeletal muscle. Our findings highlight the temporal relevance of protein phosphorylations and suggest that synchronized contributions of multiple signaling pathways form part of the circuitry for propagating information to insulin effector sites.
HostingRepositoryPRIDE
AnnounceDate2025-02-13
AnnouncementXMLSubmission_2025-02-13_09:19:10.052.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Turewicz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-30 04:09:08ID requested
12025-02-13 09:19:10announced
Publication List
10.1038/S41467-025-56335-6;
Keyword List
submitter keyword: Phosphoproteomics, Donor variability, Insulin signaling,Skeletal muscle, mRNA splicing
Contact List
Hadi Al-Hasani
contact affiliationInstitute for Clinical Biochemistry and Pathobiochemistry German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf Germany
contact emailhadi.al-hasani@ddz.de
lab head
Michael Turewicz
contact affiliationInstitute for Clinical Biochemistry and Pathobiochemistry German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich-Heine-University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf Germany
contact emailmichael.turewicz@ddz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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