PXD043423

PXD043423 is an original dataset announced via ProteomeXchange.

Title	Co-immunoprecipitation of AtNAA50 binding partners
Description	In humans and plants, 40 % of the proteome is co-translationally acetylated at the N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex consists of the catalytic subunit NAA10 and the ribosome-anchoring subunit NAA15. The regulatory subunit HYPK and the acetyltransferase NAA50 join this complex in humans. Even though both are conserved in Arabidopsis thaliana, only AtHYPK is known to interact with AtNatA. In order to verify the interaction between AtNAA50 and the core NatA subunits and to identify novel AtNAA50 interactors, wildtype leaf protein extracts were mixed with a specific antiserum against AtNAA50 (Armbruster et al., 2020). Subsequently, protein A/G beads were used to precipitate antibody-bound AtNAA50 and its interaction partners. As a negative control, protein A/G beads were incubated with leaf protein extracts lacking the specific antibody against AtNAA50.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:07.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-30 03:05:04	ID requested
1	2024-03-04 23:07:25	announced
⏵ 2	2024-10-22 06:32:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, pulldown, N-terminal protein acetylation, NAA50

Thomas Ruppert
contact affiliation	University Heidelberg, ZMBH Im Neuenheimer Feld 282 69120 Heidelberg Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Marcin Luzarowski
contact affiliation	University of Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD043423

PRIDE project URI

• PRIDE
  1. PXD043423
     1. Label: PRIDE project
     2. Name: Co-immunoprecipitation of AtNAA50 binding partners