PXD043407

PXD043407 is an original dataset announced via ProteomeXchange.

Title	High-throughput screening and proteomic characterization of compounds targeting myeloid-derived suppressor cells
Description	Myeloid-derived suppressor cells are a heterogeneous cell population of incompletely differentiated immune cells. They are known for suppressing T cell activity and are implicated in multiple chronical diseases, which makes them an attractive drug target for the pharmaceutical industry. Here, we differentiated mouse MDSC from a progenitor cell line and used quantitative (phospho)proteomics to quantify more than 7,000 proteins and phosphorylation sites that enable the characterization of MDSC on a molecular level. Based on this differentiation protocol, we investigated the effects of the well-studied MDSC drugs Entinostat and Mocetinostat on a proteomewide level and established a high-throughput drug screening platform. We assessed the effects on T cell proliferation and INF-γ secretion of ~21,000 small molecules in a MDSC/T cell coculture setup. The most promising candidates were further validated in a screening setup using human MDSC. Finally, a proteomics experiment showed the significant upregulation of several proteins associated with the reduction of reactive oxygen species, suggesting the potential mode of action of this compound.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:53:27.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Krumm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-29 12:44:04	ID requested
1	2023-08-14 23:53:08	announced
⏵ 2	2023-11-14 06:53:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: MDSC,proteomics, screening, PTM, phosphorylation

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, TU Munich
contact email	kuster@tum.de
lab head
Johannes Krumm
contact affiliation	Chair of Proteomics and Bioanalytics, TU Munich
contact email	johannes.krumm@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043407

PRIDE project URI

• PRIDE
  1. PXD043407
     1. Label: PRIDE project
     2. Name: High-throughput screening and proteomic characterization of compounds targeting myeloid-derived suppressor cells