PXD043394 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets |
Description | Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-27 |
AnnouncementXML | Submission_2024-02-27_06:57:33.517.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD043394 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Céline LECLERCQ |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | L-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-29 06:11:24 | ID requested | |
⏵ 1 | 2024-02-27 06:57:33 | announced | |
Publication List
Keyword List
submitter keyword: weaned piglet |
Ulva lactuca |
muscle |
transcriptomics |
proteomics |
Contact List
Céline LECLERCQ |
contact affiliation | LIST-ERIN-BEAP Bascharage1 Luxembourg |
contact email | celine.leclercq@list.lu |
lab head | |
Céline LECLERCQ |
contact affiliation | LIST-ERIN-ERTP-BEAP-HAU platform |
contact email | celine.leclercq@list.lu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043394
- Label: PRIDE project
- Name: Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets