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PXD043394

PXD043394 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets
DescriptionSeaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.
HostingRepositoryPRIDE
AnnounceDate2024-02-27
AnnouncementXMLSubmission_2024-02-27_06:57:33.517.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD043394
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCéline LECLERCQ
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListL-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-29 06:11:24ID requested
12024-02-27 06:57:33announced
Publication List
10.1038/S41598-024-55462-2;
10.6019/PXD043394;
Keyword List
submitter keyword: weaned piglet
Ulva lactuca
muscle
transcriptomics
proteomics
Contact List
Céline LECLERCQ
contact affiliationLIST-ERIN-BEAP Bascharage1 Luxembourg
contact emailceline.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliationLIST-ERIN-ERTP-BEAP-HAU platform
contact emailceline.leclercq@list.lu
dataset submitter
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Dataset FTP location
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