PXD043394

PXD043394 is an original dataset announced via ProteomeXchange.

Title	Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets
Description	Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.
HostingRepository	PRIDE
AnnounceDate	2024-02-27
AnnouncementXML	Submission_2024-02-27_06:57:33.517.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043394
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	L-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-29 06:11:24	ID requested
⏵ 1	2024-02-27 06:57:33	announced

10.1038/S41598-024-55462-2;

10.6019/PXD043394;

submitter keyword: weaned piglet

Ulva lactuca

muscle

transcriptomics

proteomics

Céline LECLERCQ
contact affiliation	LIST-ERIN-BEAP Bascharage1 Luxembourg
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-BEAP-HAU platform
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043394
     1. Label: PRIDE project
     2. Name: Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets