PXD043393

PXD043393 is an original dataset announced via ProteomeXchange.

Title	Dietary Laminaria digitata and enzymatic supplementation on the ileum function on weaned piglets: a combined proteomics and metabolomics analysis
Description	Laminaria digitata is a brown seaweed with prebiotic properties that has the potential to improve the response of weaned piglets to nutritional stress. However, its cell wall polysaccharides are not digested by the endogenous enzymes of monogastric animals. Alginate lyase has shown promise in degrading them under in vitro conditions. The objective of this study is to evaluate the effect of a 10% incorporation of L. digitata, and alginate lyase supplementation on the ileum proteome and metabolome, in a hypothesis generating approach. Control piglets increased the use of glucose as an enteric source of energy, demonstrated by the higher abundance of PKLR and PCK2 proteins and the lower concentration of glucose found in the tissue. Furthermore, seaweed inclusion promoted an increased abundance of proteins related with improved enterocyte structural integrity (ACTBL2, CRMP1, FLII, EML2 and MYLK), peptidase activity (NAALADL1, CAPNS1) and anti-inflammatory activity (C3), demonstrating improved intestinal function. Coherently, they lowered the abundance of apoptosis (ERN2) and proteolytic (DPP4) proteins. Alginate lyase supplementation seems to magnify the baseline effects of feeding the seaweed alone, by increasing the number of differential proteins in the same pathways, possibly as a consequence of increased intracellular nutrient release.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:21.947.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043393
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	L-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-29 05:35:06	ID requested
1	2023-09-29 07:05:00	announced
⏵ 2	2023-11-14 08:57:22	announced	2023-11-14: Updated project metadata.

10.6019/PXD043393;

submitter keyword: proteomics, metabolomics,piglet, ileum, Laminaria digitata

Céline LECLERCQ
contact affiliation	LIST-ERIN-BEAP Bascharage1 Luxembourg
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-BEAP-HAU platform
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043393
     1. Label: PRIDE project
     2. Name: Dietary Laminaria digitata and enzymatic supplementation on the ileum function on weaned piglets: a combined proteomics and metabolomics analysis