PXD043390

PXD043390 is an original dataset announced via ProteomeXchange.

Title	Alpha-glucans from bacterial necromass indicate an intra-population loop within the marine carbon cycle
Description	Phytoplankton blooms provoke bacterioplankton blooms, from which bacterial biomass (necromass) is released via increased zooplankton grazing and viral lysis. While bacterial consumption of algal biomass during blooms is wellstudied, little is known about the concurrent recycling of these substantial amounts of bacterial necromass. We demonstrate that bacterial biomass, such as bacterial alpha-glucan storage polysaccharides, generated from the consumption of algal organic matter, is reused and thus itself a major bacterial carbon source in vitro and during a diatom-dominated bloom. We highlight conserved enzymes and binding proteins of dominant bloom-responder clades that are presumably involved in the recycling of bacterial alpha-glucan by members of the bacterial community. We furthermore demonstrate that the corresponding protein machineries can be specifically induced by extracted alpha-glucan-rich bacterial polysaccharide extracts. This recycling of bacterial necromass likely constitutes a large-scale intra-population energy conservation mechanism that keeps substantial amounts of carbon in a dedicated part of the microbial loop.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:39:09.756.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Irena Beidler
SpeciesList	scientific name: Muricauda sp. MAR_2010_75; NCBI TaxID: 1250232; scientific name: Polaribacter sp. Hel_I_88; NCBI TaxID: 1250006;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-29 03:40:35	ID requested
1	2024-05-14 02:09:29	announced
⏵ 2	2024-10-22 06:39:10	announced	2024-10-22: Updated project metadata.

10.1038/S41467-024-48301-5;

submitter keyword: phytoplankton, GH13, glucan turnover, polysaccharides, global carbon cycle, β-glucan, Bacteroidota, marine bacteria, Polaribacter strain Hel_I_88, Muricauda strain MAR_2010_75,α-glucan, glycogen, laminarin, Flavobacteriia

Thomas Schweder
contact affiliation	University of Greifswald
contact email	thomas.schweder@uni-greifswald.de
lab head
Irena Beidler
contact affiliation	University of Greifswald, Department Pharmaceutical Biotechnology
contact email	irena.beidler@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD043390

PRIDE project URI

• PRIDE
  1. PXD043390
     1. Label: PRIDE project
     2. Name: Alpha-glucans from bacterial necromass indicate an intra-population loop within the marine carbon cycle