PXD043375

PXD043375 is an original dataset announced via ProteomeXchange.

Title	A comprehensive evaluation of the Ewing sarcoma surface and global proteomes reveals actionable immunotherapeutic targets.
Description	Ewing sarcoma (EwS) is the second most common bone sarcoma in children, with 1 case per 1.5 million in the United States. While the survival rate of patients diagnosed with localized disease is ~70%, this decreases to ~30% for patients with metastatic disease and only ~10% for treatment refractory disease, which remain unchanged for decades. Clearly, new therapeutic strategies are urgently needed for metastatic/refractory EwS. In this study, 19 unique EwS patient or cell line-derived xenografts were analyzed using proteomics to identify surface proteins with potential for immunotherapeutic targeting. Plasma membranes were enriched from each model via density gradient ultracentrifugation and compared to a reference standard consisting of 12 immortalized non-EwS cell lines prepared in a similar manner. In parallel, global proteome analysis was carried out on each model and reference controls to compliment the surfaceome data. All models were analyzed by Tandem Mass Tags (TMT)-based mass spectrometry to quantify identified proteins.
HostingRepository	PRIDE
AnnounceDate	2023-09-29
AnnouncementXML	Submission_2023-09-29_07:50:10.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gian LucaNegri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 13:25:30	ID requested
⏵ 1	2023-09-29 07:50:10	announced

Dataset with its publication pending

submitter keyword: Ewing sarcoma, surfaceome

GreggMorin
contact affiliation	Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver V5Z 1L3, Canada
contact email	gmorin@bcgsc.ca
lab head
Gian LucaNegri
contact affiliation	gsc
contact email	gnegri@bcgsc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD043375

PRIDE project URI

• PRIDE
  1. PXD043375
     1. Label: PRIDE project
     2. Name: A comprehensive evaluation of the Ewing sarcoma surface and global proteomes reveals actionable immunotherapeutic targets.