PXD043373

PXD043373 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiles of bona fide RNA-binding proteins across E. coli growth curve in M9LB media.
Description	Dynamic interactions between RNA and RNA-binding proteins (RBPs) regulate a broad spectrum of bacterial functions. Here, we have characterised how the RBPome is dynamically rewired during the E. coli life cycle. We applied Orthogonal Organic Phase Separation (OOPS) coupled with an RNase digestion step to shortlist bona fide bacterial RBPs and assessed whether proteins bound RNA differentially express at different cell proliferation stages.
HostingRepository	PRIDE
AnnounceDate	2024-03-20
AnnouncementXML	Submission_2024-03-20_09:41:42.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eneko Villanueva
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 10:48:04	ID requested
⏵ 1	2024-03-20 09:41:42	announced

10.1038/S44320-024-00031-Y;

submitter keyword: TMT, RNA-binding proteins, OOPS,Bacterial growth curve

Prof. Kathryn S Lilley
contact affiliation	Cambridge Centre for Proteomics, Department of Biochemistry, Gleeson Building, Tennis Court Road, Cambridge, CB21QR, United Kingdom
contact email	ksl23@cam.ac.uk
lab head
Eneko Villanueva
contact affiliation	University of Cambridge
contact email	ev318@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD043373

PRIDE project URI

• PRIDE
  1. PXD043373
     1. Label: PRIDE project
     2. Name: Proteomic profiles of bona fide RNA-binding proteins across E. coli growth curve in M9LB media.