PXD043372

PXD043372 is an original dataset announced via ProteomeXchange.

Title	Zika virus remodelled ER membranes contain an array of proviral factors involved in redox pathways
Description	Zika virus (ZIKV) has emerged as a global health issue, yet neither antiviral therapy nor vaccine are available. ZIKV is an enveloped RNA virus, replicating in the cytoplasm in close association with ER membranes with the help of host cell factors that are poorly characterized. Here, we isolated ER membranes from ZIKV-infected cells and determined their proteome. 46 host cell factors were enriched in ZIKV remodeled membranes, several of these proteins having a role in redox pathways. 4 proteins were characterized in detail. Thioredoxin Reductase 1 (TXNRD1) contributing to folding of disulfide bond containing proteins and modulating ZIKV secretion; aldo-keto reductase family 1 member C3 (AKR1C3), regulating capsid protein abundance and thus, ZIKV assembly; biliverdin reductase B (BLVRB) involved in ZIKV induced lipid peroxidation and promoting stability of non-structural proteins having transmembrane domains; Adenosylhomocysteinase (AHCY) promoting m6A methylation of ZIKV RNA and thus immune evasion. These results highlight the involvement of redox enzymes in ZIKV life cycle and their recruitment to virally remodeled ER membranes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:14:08.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quirin Emslander
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 10:01:47	ID requested
1	2023-11-14 03:54:02	announced
⏵ 2	2024-10-22 06:14:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Endoplasmatic Reticulum,Zika, Redox Pathway

Andreas Pichlmair
contact affiliation	Institute of Virology, Technical University of Munich
contact email	andreas.pichlmair@tum.de
lab head
Quirin Emslander
contact affiliation	TUM
contact email	quirin.emslander@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043372
     1. Label: PRIDE project
     2. Name: Zika virus remodelled ER membranes contain an array of proviral factors involved in redox pathways