PXD043371 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Broadcasting ERK activation in the early Drosophila embryo |
Description | The highly conserved ERK cascade controls development and physiology and is genetically deregulated in human birth defects, neurocognitive disorders, and cancers. While the list of the known ERK functions in different tissues and organisms is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here we show how new ERK functions can be systematically identified using targeted perturbations and global readouts of cell signaling systems. Our experimental model is the early Drosophila embryo, where ERK activation at the embryonic poles has thus far only been associated with transcriptional patterning of the terminal structures of the future larva. Based on the phosphoproteomic profiling after acute optogenetic perturbations of ERK signaling, we demonstrate that ERK activation at the poles is also critical for maintaining the speed and synchrony of embryonic cleavage cell cycles throughout the embryo. The presented approach sets the stage for a more complete understanding of signaling systems in health and disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:57:39.380.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Audrey Zhu |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-28 09:56:03 | ID requested | |
1 | 2024-09-03 12:15:44 | announced | |
⏵ 2 | 2024-10-22 06:57:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: ERK signaling,Phosphoproteomics, optogenetics, cell cycle |
Contact List
Martin Helmut Wuhr |
contact affiliation | Wuhr Lab, Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, USA |
contact email | wuhr@princeton.edu |
lab head | |
Audrey Zhu |
contact affiliation | Princeton University |
contact email | awzhu@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043371
- Label: PRIDE project
- Name: Broadcasting ERK activation in the early Drosophila embryo