PXD043363

PXD043363 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic landscapes provide insights into mammalian preimplantation development
Description	Proteins play a crucial role in the central dogma as the end product of gene expression. However, a deep proteomic landscape is still the missing puzzle piece of the comprehensive understanding of mammalian preimplantation development. Here, using our recently developed Comprehensive Solution of Ultrasensitive Proteome Technology (CS-UPT), we measured the proteomic profiles of oocytes and early embryos for ~5000 proteins in humans and over 6200 proteins in mice. Comparative analysis identified divergent proteomic dynamics before and around zygotic genome activation (ZGA) between the two species. Integrative analysis with transcriptome and translatome data revealed extensive and stage-specific protein turnover events. Remarkably, our comprehensive multi-omics analysis indicates that ZGA transcripts significantly contribute to protein accumulation at the blastocyst stage. Additionally, we experimentally confirmed several pf these key regulators are essential for early mouse development. Our data provide a rich resource for mammalian preimplantation development and laid a solid foundation for future investigations.
HostingRepository	PRIDE
AnnounceDate	2025-11-27
AnnouncementXML	Submission_2025-11-27_13:04:08.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Gu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 00:22:08	ID requested
⏵ 1	2025-11-27 13:04:09	announced

Zhu W, Meng J, Li Y, Gu L, Liu W, Li Z, Shen Y, Shen X, Wang Z, Wu Y, Wang G, Zhang J, Zhang H, Yang H, Dong X, Wang H, Huang X, Sun Y, Li C, Mu L, Liu Z, Comparative proteomic landscapes elucidate human preimplantation development and failure. Cell, 188(3):814-831.e21(2025) [pubmed]

10.1016/j.cell.2024.12.028;

submitter keyword: Protein turnover, Mouse preimplantation development, Multi-omics analysis, Zygotic genome activation (ZGA),Ultrasensitive proteomics, Cross species analysis

Chen Li
contact affiliation	Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine
contact email	cli@shsmu.edu.cn
lab head
Lei Gu
contact affiliation	Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine
contact email	summerrushing@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD043363

PRIDE project URI

• PRIDE
  1. PXD043363
     1. Label: PRIDE project
     2. Name: Comparative proteomic landscapes provide insights into mammalian preimplantation development