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PXD043342

PXD043342 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRAVER1 interconnects lethal EMT and miR/RISC activity by the control of alternative splicing
DescriptionThe RAVER1 protein was proposed to serve as a co-factor in guiding the PTBP-dependent control of alternative splicing (AS). Whether RAVER1 solely acts in concert with PTBPs and how it affects cancer cell fate remained elusive. Here we provide the first comprehensive investigation of RAVER1-controlled AS in cancer cell models and reveal a pro-oncogenic role of RAVER1 in tumor growth. This unravels that RAVER1 guides AS in synergy with PTBPs but more prominently serves PTBP1-independent roles in splicing. In cancer cells, one major function of RAVER1 is the control of proliferation and apoptosis, which involves the modulation of AS events within the miR/RISC pathway. Associated with this regulatory role, RAVER1 antagonizes lethal, TGFB-driven epithelial-mesenchymal-transition (EMT) by limiting TGFB signaling. RAVER1-modulated splicing events affect the insertion of protein interaction modules in factors guiding miR/RISC-dependent gene silencing. Most prominently, in all three human TNRC6 proteins, RAVER1 controls AS of GW-enriched motifs, which are essential for AGO2-binding. Disturbance of RAVER1-guided AS events in TNRC6 proteins and other facilitators of miR/RISC activity compromise miR/RISC activity which is essential to restrict TGFB signaling and lethal EMT.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:20:07.027.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD043342
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristian Ihling
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-27 07:16:25ID requested
12024-01-10 23:09:48announced
22024-10-22 06:20:07announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD043342;
Keyword List
submitter keyword: cancer, EMT,RAVER1, TGFB, microRNA, PTBP, alternative splicing, RISC
Contact List
Stefan Hüttelmaier
contact affiliationMartin Luther University (MLU) Institute of Molecular Medicine (IMM) Sect. Molecular Cell Biology
contact emailstefan.huettelmaier@medizin.uni-halle.de
lab head
Christian Ihling
contact affiliationMLU Halle, Inst. f. Pharmacy
contact emailchristian.ihling@pharmazie.uni-halle.de
dataset submitter
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Dataset FTP location
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Repository Record List
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