PXD043335

PXD043335 is an original dataset announced via ProteomeXchange.

Title	Osteogenic human MSC-derived extracellular vesicles regulate MSC activity and osteogenic differentiation and promote bone regeneration in a rat calvarial defect model
Description	The objective of this study was to explore the characteristics and roles of extracellular vesicles (EVs) obtained from human mesenchymal stem cells (MSCs) under two culture conditions: undifferentiated MSCs (Naïve-EVs) and MSCs undergoing early-stage osteogenesis (Osteo-EVs). Additionally, the study aimed to evaluate the potential of Osteo-EVs to induce bone formation for the purpose of regenerating bone defects in rat calvaria. To accomplish these goals, EVs from both groups were isolated using a technique called size-exclusion chromatography. The isolated EVs were then subjected to various analyses to gain insights into their properties. This included examining their size distribution, morphology, flow cytometry analysis, and proteome profiling using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with Label Free Quantification.
HostingRepository	PRIDE
AnnounceDate	2024-01-10
AnnouncementXML	Submission_2024-01-10_05:31:45.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niyaz Alsharabi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-27 04:03:34	ID requested
⏵ 1	2024-01-10 05:31:46	announced

Dataset with its publication pending

submitter keyword: Mesenchymal stem cells, Rat calvarial defects.,Extracellular vesicles, Bone regeneration, Naïve-EVs, Osteo-EVs

Kamal Mustafa
contact affiliation	TOR center, Department of Dentistry, Uinversity Of Bergen, Norway
contact email	Kamal.Mustafa@uib.no
lab head
Niyaz Alsharabi
contact affiliation	University of Bergen
contact email	N.Al-Sharabi@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043335

PRIDE project URI

• PRIDE
  1. PXD043335
     1. Label: PRIDE project
     2. Name: Osteogenic human MSC-derived extracellular vesicles regulate MSC activity and osteogenic differentiation and promote bone regeneration in a rat calvarial defect model