PXD043328

PXD043328 is an original dataset announced via ProteomeXchange.

Title	Primary Cilia-Mediated Regulation of Microglial Secretion in Alzheimer's Disease
Description	Proteomic approaches revealed that primary cilia, small hair-like structures found on cells, played a role in the regulation of microglial secretory function. Notably, primary cilia were transiently observed in less than 10% of microglia, and their presence was significantly reduced in microglia from Alzheimer's disease (AD) mice. We observed significant changes in the expression and distribution of secretomes after inhibiting the primary cilia gene intraflagellar transport particle 88 (Ift88) in microglia. Intriguingly, inhibiting primary cilia in the SEM of AD mice resulted in the expansion of extracellular amyloid plaques and damage to adjacent neurites. These results indicate that DAM-like microglia are present in the septumLS, a critical target region for hippocampal nerve bundles, and that the primary ciliary signaling system regulates microglial secretion, affecting extracellular proteostasis. Age-related primary ciliopathy probably contributes to the selective sensitivity of microglia, thereby exacerbating AD. To elucidate the dynamic changes in microglial proteomics resulting from silenced Ift88 and Aβ treatment, we performed an analysis of BV2 cell lysates and extracellular vesicles (EVs) by downregulating Ift88 expression. To investigate the specific proteomic alterations in EVs associated with CD63 and CD81, we conducted a proteomic characterization of CD63- or CD81-bound EVs in the lysate and EVs secreted from BV2 cells transfected with siIft88 and treated with Aβ, utilizing co-immunoprecipitation (Co-IP) with anti-CD63 or anti-CD81 antibodies.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_14:54:19.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaemyung Jang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 22:45:01	ID requested
⏵ 1	2024-01-26 14:54:20	announced

Yeo S, Jang J, Jung HJ, Lee H, Choe Y, Primary cilia-mediated regulation of microglial secretion in Alzheimer's disease. Front Mol Biosci, 10():1250335(2023) [pubmed]

10.3389/fmolb.2023.1250335;

submitter keyword: Microglia, Primary Cilia,Alzheimer's disease

Youngshik Choe
contact affiliation	Korea Brain Research Institute, Daegu, 41068, Korea
contact email	dallarae@kbri.re.kr
lab head
Jaemyung Jang
contact affiliation	Korea Brain Research Institute
contact email	piloter2@kbri.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043328

PRIDE project URI

• PRIDE
  1. PXD043328
     1. Label: PRIDE project
     2. Name: Primary Cilia-Mediated Regulation of Microglial Secretion in Alzheimer's Disease