PXD043322
PXD043322 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systematic discovery of linear ubiquitination mediated neo-protein-protein interactions across mouse tissues |
Description | HOIP truncations proteins GST-PUB, GST-NZF, GST-UBA and GST-RBR-LDD were purified, GST was used as a negative control. The tissue lysates from the C57BL/6 mice were incubated with glutathione-S-transferase (GST) beads which bound to the GST-HOIP truncation proteins beforehand. The pull-down component were separated by SDS-PAGE and stained with Coomassie brilliant blue. The band were cut off and subject to mass spectrometry. |
HostingRepository | iProX |
AnnounceDate | 2024-02-28 |
AnnouncementXML | Submission_2025-05-21_18:59:56.031.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Li |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-06-26 19:27:41 | ID requested | |
1 | 2024-02-28 02:44:42 | announced | |
⏵ 2 | 2025-05-21 18:59:56 | announced | 2025-05-22: Add Publication. |
Publication List
Fu Y, Li L, Zhang X, Deng Z, Wu Y, Chen W, Liu Y, He S, Wang J, Xie Y, Tu Z, Lyu Y, Wei Y, Wang S, Cui CP, Liu CH, Zhang L, Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis. Nat Commun, 15(1):2974(2024) [pubmed] |
Keyword List
submitter keyword: Quantitative HOIP interactome profiles, mouse tissues |
Contact List
Lingqiang Zhang | |
---|---|
contact affiliation | State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics |
contact email | zhanglq@nic.bmi.ac.cn |
lab head | |
Lei Li | |
contact affiliation | National Center for Protein Sciences (Beijing) |
contact email | lilei_birm@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |