PXD043320

PXD043320 is an original dataset announced via ProteomeXchange.

Title	The proteome and lipidome of extracellular vesicles from Haemonchus contortus to underpin explorations of host-para-site cross-talk
Description	Many parasitic worms have a major adverse impact in human and animal populations worldwide due to the chronicity of their infections. There is a growing body of evidence indicating that extracellular vesicles (EVs) are intimately involved in modulating (suppressing) inflamma-tory/immune host responses and parasitism. As one of the most pathogenic nematodes of livestock animals, Haemonchus contortus is an ideal model system for EV exploration. Here, employing a multi-step enrichment process (in vitro culture, followed by ultracentrifugation, size exclusion and filtration), we enriched EVs from H. contortus and undertook the first comprehensive (qualitative and quantitative) multi-omic investigation of EV proteins and lipids using advanced liquid chro-matography–mass spectrometry and informatic methods. We identified and quantified 561 proteins and 446 lipids in EVs, and compared these molecules with those of adult worms. We identified unique molecules in EVs, such as proteins linked to lipid transportation and lipid species (i.e. glyc-erophospholipids and sphingolipids) associated with signalling, indicating an involvement of these molecules in parasite-host cross-talk. This work provides a solid starting point to explore the func-tional roles of EV-specific proteins and lipids in modulating parasite-host cross talk, and the pro-spect of finding ways of disrupting or interrupting this relationship to suppress or eliminate para-site infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:28.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 19:23:50	ID requested
1	2023-08-06 16:19:38	announced
⏵ 2	2023-11-14 09:04:31	announced	2023-11-14: Updated project metadata.

Wang T, Koukoulis TF, Vella LJ, Su H, Purnianto A, Nie S, Ang CS, Ma G, Korhonen PK, Taki AC, Williamson NA, Reid GE, Gasser RB, to Underpin Explorations of Host-Parasite Cross-Talk. Int J Mol Sci, 24(13):(2023) [pubmed]

submitter keyword: Parasitic nematode

Haemonchus contortus

Proteomics

Lipidomics

LC-MS/MS

Host-parasite interactions

Robin Beat Gasser
contact affiliation	Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043320

PRIDE project URI

• PRIDE
  1. PXD043320
     1. Label: PRIDE project
     2. Name: The proteome and lipidome of extracellular vesicles from Haemonchus contortus to underpin explorations of host-para-site cross-talk