PXD043318

PXD043318 is an original dataset announced via ProteomeXchange.

Title	Deep-sea in situ and laboratory proteomics provide insights into the sulfur metabolism characteristics of a novel deep-sea bacterium, Pseudodesulfovibrio serpens sp. nov.
Description	Sulfate-reducing bacteria (SRB) are ubiquitously distributed across various biospheres and play key roles in global sulfur and carbon cycles. However, few deep-sea SRB have been cultivated and studied in situ, limiting our understanding of the true metabolism of SRB in the deep biosphere. Here, we firstly clarified the high abundance of SRB in deep-sea sediments via the operational taxonomic units (OTU) sequencing analysis. We have successfully isolated a sulfate-reducing bacterium (strain zrk46) from a cold seep sediment, by using an enriched medium supplemented with sulfate. Our genomic, physiological and phylogenetic analyses indicate that strain zrk46 is a novel species, which we propose be named: Pseudodesulfovibrio serpens. Based on the combined results from growth assays and proteomic analyses, we found that supplementation with sulfate (SO42-), thiosulfate (S2O32-), or sulfite (SO32-) promoted the growth of strain zrk46 by facilitating energy production through the dissimilatory sulfate reduction with the auxiliary functions of heterodisulfide reductases, ferredoxins, and nitrate reduction associated proteins, which were coupled to the oxidation of environmental organic matter in both laboratory and deep-sea in situ conditions. Moreover, metatranscriptomic results have also confirmed the dissimilatory sulfate reduction of deep-sea SRB in in situ environment, which might be coupled to the methane oxidation of anaerobic methanotrophic archaea (ANME-2). Overall, these findings expand our understanding of deep-sea SRB, while highlighting their importance for deep-sea sulfur and carbon cycles.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:20:58.771.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sun lab sun lab
SpeciesList	scientific name: environmental samples
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 18:21:44	ID requested
⏵ 1	2026-02-22 16:20:59	announced

10.1016/j.isci.2024.110095;

Wang C, Zheng R, Sun C, Multi-omics analyses provide insights into the sulfur metabolism of a novel deep-sea sulfate-reducing bacterium. iScience, 27(6):110095(2024) [pubmed]

submitter keyword: Sulfate-reducing bacteria

cultivation

deep sea

sulfur metabolism

in situ

proteomics

metatranscriptomics

Chaomin Sun
contact affiliation	Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
contact email	sunchaomin@qdio.ac.cn
lab head
sun lab sun lab
contact affiliation	institute of oceanology
contact email	1365105894@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043318
     1. Label: PRIDE project
     2. Name: Deep-sea in situ and laboratory proteomics provide insights into the sulfur metabolism characteristics of a novel deep-sea bacterium, Pseudodesulfovibrio serpens sp. nov.