PXD043291

PXD043291 is an original dataset announced via ProteomeXchange.

Title	Dissecting ubiquitylation and DNA damage response pathways in the yeast Saccharomyces cerevisiae using a proteome-wide approach
Description	The proper function of DNA damage response pathways (DDR) is essential to protect eukaryotic cells from the accumulation of DNA damage and genome instability, a major cause of cancer and other diseases in humans. Ubiquitylation orchestrates the regulation of DDR events.Blaszczak et al. use quantitative proteomics and a reporter system based on the NanoLuc luciferase to study ubiquitylation and protein abundance changes upon genotoxic stress in yeast. The authors point out proteins that may be regulated by ubiquitylation during the DDR.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:15:14.802.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043291
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ewa Blaszczak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	6220 Time-of-Flight LC/MS; MALDI Synapt MS; Synapt MS; Q Exactive HF; LCQ Classic; TripleTOF 5600; 6410 Triple Quadrupole LC/MS; Q Exactive; LTQ Orbitrap; 4800 Proteomics Analyzer; 6340 Ion Trap LC/MS; Orbitrap Fusion Lumos; LTQ; LTQ Orbitrap Velos; Q TRAP; Q-Tof Ultima; maXis; LTQ FT; autoflex; ultraflex; QSTAR; 4700 Proteomics Analyzer; Orbitrap Fusion; 6520A Quadrupole Time-of-Flight LC/MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 01:46:48	ID requested
⏵ 1	2024-10-08 11:15:15	announced

10.6019/PXD043291;

10.1016/J.MCPRO.2023.100695;

submitter keyword: ubiquitylation, IMAC, NanoLuc, qunatitative proteomics, anchor-away, degradation, SILAC, proteasome, MMS, genotoxic stress,ubiquitin, DNA damage response

Ewa Blaszczak
contact affiliation	Department of Biochemistry and Molecular Biology, Faculty of Medical Sciences, Medical University of Lublin
contact email	ewa.blaszczak1@umlub.pl
lab head
Ewa Blaszczak
contact affiliation	Department of Biochemistry and Molecular Biology, Faculty of Medical Sciences, Medical University of Lublin
contact email	ewa.blaszczak1@umlub.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043291
     1. Label: PRIDE project
     2. Name: Dissecting ubiquitylation and DNA damage response pathways in the yeast Saccharomyces cerevisiae using a proteome-wide approach