PXD043290

PXD043290 is an original dataset announced via ProteomeXchange.

Title	Deep proteomic analysis of circulating large/small extracellular vesicle enriched fraction based on data-independent acquisition mass spectrometry
Description	Proteins in the plasma/serum mirror an individual’s physiology. Circulating extracellular vesicle (EVs) proteins constitute a large portion of the plasma/serum proteome. Thus, deep and unbiased proteomic analysis of circulating plasma/serum extracellular vesicles holds promise for discovering disease biomarkers as well as revealing disease mechanisms. We established a workflow for simple, deep, and reproducible proteome analysis of both serum large and small EVs enriched fractions by ultracentrifugation plus 4D-DIA-MS. In our cohort study of obstetric antiphospholipid syndrome (OAPS), 4270 and 3328 proteins were identified from large and small EVs enriched fractions respectively. Both of them revealed known or new pathways related to OAPS. Increased levels of von Willebrand factor (VWF) and insulin receptor (INSR) were identified biomarker features, which sheds light on hypercoagulability and insulin signaling in disease progression. Our workflow for deep quantitative proteome of EV enriched fraction combined with targeted validations will significantly promote our understanding of plasma/serum-based disease mechanisms and generate new biomarkers.
HostingRepository	iProX
AnnounceDate	2023-06-26
AnnouncementXML	Submission_2024-02-22_02:18:13.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haohao Tang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 01:45:01	ID requested
1	2023-06-26 01:45:11	announced
⏵ 2	2024-02-22 02:18:14	announced	2024-02-22: Update information.

Tian W, Shi D, Zhang Y, Wang H, Tang H, Han Z, Wong CCL, Cui L, Zheng J, Chen Y, Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions. Commun Biol, 7(1):99(2024) [pubmed]

submitter keyword: proteomic, arge/small extracellular vesicle, data-independent acquisition

Yang Chen
contact affiliation	Peking University Health Science Center
contact email	chenyang1816185048@bjmu.edu.cn
lab head
Haohao Tang
contact affiliation	Peking University Health Science Center
contact email	Tanghaohao0987@163.com
dataset submitter

iProX dataset URI