PXD043268

PXD043268 is an original dataset announced via ProteomeXchange.

Title	Chronotoxici-plate Containing Rhythmic Liver Organoids for Drug Screening
Description	The circadian clock coordinates the daily rhythmicity of biological processes, and its dysregulation is associated with various human diseases. Despite the direct targeting of rhythmic genes by many best-selling and WHO essential drugs, traditional approaches can’t satisfy the need of explore multi-timepoint drug administration strategies across a wide range of drugs. Here, we developed Chronotoxici-plate, a high-throughput automated rhythmic organoid tool for chronotherapy assessment. We identified Cry1 as a rhythmic marker in DPLOs, providing insights for rapid assessment of organoid rhythmicity. Using Oxaliplatin as a representative drug, we demonstrated time-dependent variations in toxicity on the Chronotoxici-plate, highlighting the importance of considering time-dependent effects. Additionally, we observed improved development of rhythmic organoids, indicating a novel dimension in primary organoid culture. We successfully generated in vitro rhythmic primary organoids within four days, and enabled a preliminary assessment of chronotherapy's drug safety within seven days. This approach holds great promise for reducing drug side effects by optimizing administration timing and expanding the bank of available drugs.
HostingRepository	iProX
AnnounceDate	2023-06-25
AnnouncementXML	Submission_2024-06-30_20:03:04.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaqi Zhou
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	iTRAQ quantitation analysis

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-24 21:01:37	ID requested
⏵ 1	2024-06-30 20:03:04	announced

Zhou J, Huang YC, Wang W, Li J, Hou Y, Yi Z, Yang H, Hu K, Zhu Y, Wang Z, Ma S, Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation. Adv Sci (Weinh), 11(28):e2305925(2024) [pubmed]

submitter keyword: Chronotherapy, engineering liver organoid, Oxaliplatin, Cry1

Shaohua Ma
contact affiliation	Tsinghua University
contact email	ma.shaohua@sz.tsinghua.edu.cn
lab head
Jiaqi Zhou
contact affiliation	Tsinghua University
contact email	zhoujq21@mails.tsinghua.edu.cn
dataset submitter

iProX dataset URI