PXD043268
PXD043268 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chronotoxici-plate Containing Rhythmic Liver Organoids for Drug Screening |
Description | The circadian clock coordinates the daily rhythmicity of biological processes, and its dysregulation is associated with various human diseases. Despite the direct targeting of rhythmic genes by many best-selling and WHO essential drugs, traditional approaches can’t satisfy the need of explore multi-timepoint drug administration strategies across a wide range of drugs. Here, we developed Chronotoxici-plate, a high-throughput automated rhythmic organoid tool for chronotherapy assessment. We identified Cry1 as a rhythmic marker in DPLOs, providing insights for rapid assessment of organoid rhythmicity. Using Oxaliplatin as a representative drug, we demonstrated time-dependent variations in toxicity on the Chronotoxici-plate, highlighting the importance of considering time-dependent effects. Additionally, we observed improved development of rhythmic organoids, indicating a novel dimension in primary organoid culture. We successfully generated in vitro rhythmic primary organoids within four days, and enabled a preliminary assessment of chronotherapy's drug safety within seven days. This approach holds great promise for reducing drug side effects by optimizing administration timing and expanding the bank of available drugs. |
HostingRepository | iProX |
AnnounceDate | 2023-06-25 |
AnnouncementXML | Submission_2024-06-30_20:03:04.091.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiaqi Zhou |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | iTRAQ quantitation analysis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-06-24 21:01:37 | ID requested | |
⏵ 1 | 2024-06-30 20:03:04 | announced |
Publication List
Zhou J, Huang YC, Wang W, Li J, Hou Y, Yi Z, Yang H, Hu K, Zhu Y, Wang Z, Ma S, Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation. Adv Sci (Weinh), 11(28):e2305925(2024) [pubmed] |
Keyword List
submitter keyword: Chronotherapy, engineering liver organoid, Oxaliplatin, Cry1 |
Contact List
Shaohua Ma | |
---|---|
contact affiliation | Tsinghua University |
contact email | ma.shaohua@sz.tsinghua.edu.cn |
lab head | |
Jiaqi Zhou | |
contact affiliation | Tsinghua University |
contact email | zhoujq21@mails.tsinghua.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |