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PXD043268

PXD043268 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChronotoxici-plate Containing Rhythmic Liver Organoids for Drug Screening
DescriptionThe circadian clock coordinates the daily rhythmicity of biological processes, and its dysregulation is associated with various human diseases. Despite the direct targeting of rhythmic genes by many best-selling and WHO essential drugs, traditional approaches can’t satisfy the need of explore multi-timepoint drug administration strategies across a wide range of drugs. Here, we developed Chronotoxici-plate, a high-throughput automated rhythmic organoid tool for chronotherapy assessment. We identified Cry1 as a rhythmic marker in DPLOs, providing insights for rapid assessment of organoid rhythmicity. Using Oxaliplatin as a representative drug, we demonstrated time-dependent variations in toxicity on the Chronotoxici-plate, highlighting the importance of considering time-dependent effects. Additionally, we observed improved development of rhythmic organoids, indicating a novel dimension in primary organoid culture. We successfully generated in vitro rhythmic primary organoids within four days, and enabled a preliminary assessment of chronotherapy's drug safety within seven days. This approach holds great promise for reducing drug side effects by optimizing administration timing and expanding the bank of available drugs.
HostingRepositoryiProX
AnnounceDate2023-06-25
AnnouncementXMLSubmission_2024-06-30_20:03:04.091.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiaqi Zhou
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentiTRAQ quantitation analysis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-24 21:01:37ID requested
12024-06-30 20:03:04announced
Publication List
Zhou J, Huang YC, Wang W, Li J, Hou Y, Yi Z, Yang H, Hu K, Zhu Y, Wang Z, Ma S, Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation. Adv Sci (Weinh), 11(28):e2305925(2024) [pubmed]
Keyword List
submitter keyword: Chronotherapy, engineering liver organoid, Oxaliplatin, Cry1
Contact List
Shaohua Ma
contact affiliationTsinghua University
contact emailma.shaohua@sz.tsinghua.edu.cn
lab head
Jiaqi Zhou
contact affiliationTsinghua University
contact emailzhoujq21@mails.tsinghua.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI