PXD043264
PXD043264 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | UV‑ARTP compound mutagenesis breeding improves macrolactin production of Bacillus siamensis and reveals metabolism changes by proteomic |
Description | Macrolactin is a compound with complex macrolide structure which only be obtained through microbiological fermentation now. It has excellent antifungal, antibacterial and antitumor activity. In this study, Bacillus siamensis YB304 was used as the research object, and a mutant Mut-K53 with stable genetic characters was selected by UV-ARTP compound mutation. The macrolactin yield was 156.5 mg/L, 395% higher than original strain. The metabolic pathway changes and the regulatory mechanism of macrolactin were analyzed by quantitative proteomics combined with parallel reaction monitoring. The results showed that the utilization of carbon sources such as sucrose was enhanced in Mut-K53; the glycolysis pathway was positively up-regulated; the amino acid metabolism pathway such as aromatic amino acids was significantly up-regulated. The production of various precursor substances such as acyl-CoA and amino acids was one of the main reasons for the increase of macrolactin production. At the same time, the key proteins of macrolactin biosynthesis are mostly up-regulated, which is the direct reason for the increase of macrolactin production. |
HostingRepository | iProX |
AnnounceDate | 2023-06-21 |
AnnouncementXML | Submission_2024-05-29_21:00:36.799.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fei Li |
SpeciesList | scientific name: Bacillus siamensis; NCBI TaxID: 659243; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-06-24 20:40:53 | ID requested | |
1 | 2023-06-24 20:41:10 | announced | |
⏵ 2 | 2024-05-29 21:00:37 | announced | 2024-05-30: Update information. |
Publication List
Yu L, Li F, Ni J, Qin X, Lai J, Su X, Li Z, Zhang M, UV-ARTP compound mutagenesis breeding improves macrolactins production of Bacillus siamensis and reveals metabolism changes by proteomic. J Biotechnol, 381():36-48(2024) [pubmed] |
Keyword List
submitter keyword: Macrolactin, UV mutagenesis, ARTP mutagenesis, Proteomics, Parallel reaction monitoring, Bacillus siamensis |
Contact List
Lian Yu | |
---|---|
contact affiliation | Guangxi University |
contact email | lianyu@gxu.edu.cn |
lab head | |
Fei Li | |
contact affiliation | Guangxi Academy of Sciences |
contact email | finylee@yeah.net |
dataset submitter |
Full Dataset Link List
iProX dataset URI |