PXD043264

PXD043264 is an original dataset announced via ProteomeXchange.

Title	UV‑ARTP compound mutagenesis breeding improves macrolactin production of Bacillus siamensis and reveals metabolism changes by proteomic
Description	Macrolactin is a compound with complex macrolide structure which only be obtained through microbiological fermentation now. It has excellent antifungal, antibacterial and antitumor activity. In this study, Bacillus siamensis YB304 was used as the research object, and a mutant Mut-K53 with stable genetic characters was selected by UV-ARTP compound mutation. The macrolactin yield was 156.5 mg/L, 395% higher than original strain. The metabolic pathway changes and the regulatory mechanism of macrolactin were analyzed by quantitative proteomics combined with parallel reaction monitoring. The results showed that the utilization of carbon sources such as sucrose was enhanced in Mut-K53; the glycolysis pathway was positively up-regulated; the amino acid metabolism pathway such as aromatic amino acids was significantly up-regulated. The production of various precursor substances such as acyl-CoA and amino acids was one of the main reasons for the increase of macrolactin production. At the same time, the key proteins of macrolactin biosynthesis are mostly up-regulated, which is the direct reason for the increase of macrolactin production.
HostingRepository	iProX
AnnounceDate	2023-06-21
AnnouncementXML	Submission_2024-05-29_21:00:36.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fei Li
SpeciesList	scientific name: Bacillus siamensis; NCBI TaxID: 659243;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-24 20:40:53	ID requested
1	2023-06-24 20:41:10	announced
⏵ 2	2024-05-29 21:00:37	announced	2024-05-30: Update information.

Yu L, Li F, Ni J, Qin X, Lai J, Su X, Li Z, Zhang M, UV-ARTP compound mutagenesis breeding improves macrolactins production of Bacillus siamensis and reveals metabolism changes by proteomic. J Biotechnol, 381():36-48(2024) [pubmed]

submitter keyword: Macrolactin, UV mutagenesis, ARTP mutagenesis, Proteomics, Parallel reaction monitoring, Bacillus siamensis

Lian Yu
contact affiliation	Guangxi University
contact email	lianyu@gxu.edu.cn
lab head
Fei Li
contact affiliation	Guangxi Academy of Sciences
contact email	finylee@yeah.net
dataset submitter

iProX dataset URI