PXD043255

PXD043255 is an original dataset announced via ProteomeXchange.

Title	Infant milk formula enriched in dairy cream brings its digestibility closer to human milk and supports intestinal health
Description	Human breast milk (HBM) is the “gold standard” for infant nutrition. When breast milk is insufficient or unavailable, infant milk formula (IMF) can provide a safe and nutritious alternative. However, IMFs often differ considerably from HBM in composition, particularly fatty acid profiles, and health function. Here, we compared the digestibility and potential health functions of IMF containing low cream (LC-) or high cream (HC-) content compared to pooled HBM. After simulated infant digestion of these samples, bioavailability of key nutrients and immunomodulatory activities were determined via cell-based in-vitro assays. A Caenorhabditis elegans (C.elegans) leaky gut model was also established to investigate effects on gut health. Distinct differences were observed in higher diversity of peptides released from HC-IMF when compared to LC-IMF during simulated digestion. Higher levels of free fatty acids were absorbed through 21-day differentiated Caco-2/HT-29MTX monolayers from HC-IMF, when compared to LC-IMF and HBM. Furthermore, the immune-modulating properties of HC-IMF appeared to be more in line with HBM than LC-IMF, as observed by comparable secretion of IL-10 and IL-1β from THP-1 cells following exposure. HC-IMF also supported significantly faster intestinal recovery following distortion, vs. LC-IMF in C.elegans. These initial observations suggest that increasing the cream content in infant milk formulation may provide added nutritional and functional benefits which are closer to that of HBM.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_10:02:16.996.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gianfranco Mamone
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-23 16:34:26	ID requested
⏵ 1	2025-05-06 10:02:17	announced

Kondrashina A, Mamone G, Giblin L, Lane JA, Infant Milk Formula Enriched in Dairy Cream Brings Its Digestibility Closer to Human Milk and Supports Intestinal Health in Pre-Clinical Studies. Nutrients, 16(18):(2024) [pubmed]

10.3390/nu16183065;

submitter keyword: CaCo-2

infant formula

infant nutrition

milk fat globule

dairy lipids

Gianfranco Mamone
contact affiliation	National Research Council, Institute of Food Science - Avellino Itale
contact email	mamone@isa.cnr.it
lab head
Gianfranco Mamone
contact affiliation	CNR ITALY
contact email	mamone@isa.cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043255
     1. Label: PRIDE project
     2. Name: Infant milk formula enriched in dairy cream brings its digestibility closer to human milk and supports intestinal health