PXD043244

PXD043244 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic profiling of mouse embryonic fibroblasts
Description	Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wildtype primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were enriched in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70RSK, and MTOR. Cell surface markers and matrisome proteins were also annotated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:38.298.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043244
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicole Verrills
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-23 08:14:07	ID requested
1	2023-10-20 04:39:22	announced
2	2023-11-14 09:06:12	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:39	announced	2024-10-22: Updated project metadata.

10.6019/PXD043244;

Chen Y, Roselli S, Panicker N, Brzozowski JS, Skerrett-Byrne DA, Murray HC, Verrills NM, Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts. Proteomics, 24(7):e2300267(2024) [pubmed]

10.1002/pmic.202300267;

submitter keyword: Phosphoproteomics, Proteomics, fibroblasts

Nicole Verrills
contact affiliation	School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, and Precision Medicine Program, Hunter Medical Research Institute, NSW, 2308, Australia
contact email	nikki.verrills@newcastle.edu.au
lab head
Nicole Verrills
contact affiliation	School of Biomedical Sciences and Pharmacy Faculty of Health and Medicine, University of Newcastle, and Cancer Research Program, Hunter Medical Research Institute, Callaghan, NSW, 2308, Australia
contact email	Nikki.Verrills@newcastle.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043244

PRIDE project URI

• PRIDE
  1. PXD043244
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic profiling of mouse embryonic fibroblasts