PXD043243

PXD043243 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis in root tip of maize under cadmium and copper exposure
Description	In this study, the metabolic adjustments performed by maize (Zea mays L) seminal roots exposed to 25 µM Cd2+ or 25 µM Cu2+ at pre-emergence are compared, focusing on the proteomic changes after metal exposure. Root width was increased, and root length was decreased after 72 h of metal treatment. Both metals induced H2O2 accumulation and lipid peroxidation in the root tip. These changes were accompanied by increases in lipoxygenase activity and 4-hydroxy-2-nonenal content. NMR spectroscopy revealed that the abundance of 38 water-soluble metabolites was significantly modified by Cd and Cu exposure; this set of metabolites comprised carboxylic acids, amino acids, carbohydrates, and unidentified phenolic compounds. Linoleic acid content significantly decreased in Cu-treated samples. The total amount of proteins detected in maize root apexes was 2,171. Gene ontology enrichment analysis of the differentially accumulated proteins was performed to detect pathways probably affected by metal additions. Both metals altered redox homeostasis, up-regulated oxylipins biosynthetic process, and shifted metabolism towards the oxidative pentose-phosphate in the root apexes. However, the methionine salvage pathway appears as a key metabolic module only under Cd stress. The integrative analysis carried out in this study suggests that most molecular features behind the reprogramming of maize root tips to cope with cadmium and copper toxicity are common, but some are not.
HostingRepository	PRIDE
AnnounceDate	2023-11-22
AnnouncementXML	Submission_2023-11-22_05:50:07.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liliana Pena
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-23 06:31:53	ID requested
⏵ 1	2023-11-22 05:50:07	announced

10.1007/S10534-023-00557-Y;

submitter keyword: proteomics,Zea mays, LC-MS/MS

Liliana Beatriz
contact affiliation	Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica, Departamento de Química Biológica. Buenos Aires, Argentina CONICET. Instituto de Química y Fisicoquímica Biológicas (IQUIFIB). Buenos Aires, Argentina
contact email	lpena@ffyb.uba.ar
lab head
Liliana Pena
contact affiliation	Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica
contact email	lpena@ffyb.uba.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD043243

PRIDE project URI

• PRIDE
  1. PXD043243
     1. Label: PRIDE project
     2. Name: Proteomic analysis in root tip of maize under cadmium and copper exposure