PXD043213

PXD043213 is an original dataset announced via ProteomeXchange.

Title	Targeting mitophagy in SRSF2 mutant hematologic malignancies
Description	Recurrent Glycogen synthase kinase-3 (GSK-3) phosphorylates multiple splicing factors, including SRSF2, and regulates the splicing of a broad range of mRNAs in human cells. Inhibition of GSK-3 disrupts splicing and promotes cell death in hematopoietic cells with heterozygous mutations in SRSF2 and not in cells with wild-type splicing factors. To characterize how GSK-3 inhibition alters the cellular proteome in SRSF2-P95H/+ cells, we have performed quantitative mass spectrometry (qMS) on K562 cells with SRSF2P95H/+ knocked into the endogenous locus (PMID 30799057) and parental K562 cells, treated with the GSK-3 inhibitor CHIR99021. We focused on the mitochondrial proteome using human proteome (UniprotKB) and mitochondrial (MitoCarta 3.0) databases for protein identification, identifying 163 mitochondrial proteins whose levels are affected by SRSF2 mutation.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:36:56.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin A Janssen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-22 09:30:55	ID requested
⏵ 1	2024-06-22 08:36:57	announced

Liu X, Devadiga SA, Stanley RF, Morrow RM, Janssen KA, Quesnel-Valli, è, res M, Pomp O, Moverley AA, Li C, Skuli N, Carroll M, Huang J, Wallace DC, Lynch KW, Abdel-Wahab O, Klein PS, A mitochondrial surveillance mechanism activated by SRSF2 mutations in hematologic malignancies. J Clin Invest, 134(12):(2024) [pubmed]

10.1172/jci175619;

ProteomeXchange project tag: Human Proteome Project

submitter keyword: mitophagy, chronic myelomonocytic leukemia (CMML),myelodysplasia, SRSF2, acute myeloid leukemia, splicing, nonsense mediated decay, GSK-3, PINK1

Douglas C. Wallace
contact affiliation	Children's Hospital of Philadelphia
contact email	wallaced1@chop.edu
lab head
Kevin A Janssen
contact affiliation	Children's Hospital of Philadelphia
contact email	kxjanssen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD043213

PRIDE project URI

• PRIDE
  1. PXD043213
     1. Label: PRIDE project
     2. Name: Targeting mitophagy in SRSF2 mutant hematologic malignancies