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PXD043213

PXD043213 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeting mitophagy in SRSF2 mutant hematologic malignancies
DescriptionRecurrent Glycogen synthase kinase-3 (GSK-3) phosphorylates multiple splicing factors, including SRSF2, and regulates the splicing of a broad range of mRNAs in human cells. Inhibition of GSK-3 disrupts splicing and promotes cell death in hematopoietic cells with heterozygous mutations in SRSF2 and not in cells with wild-type splicing factors. To characterize how GSK-3 inhibition alters the cellular proteome in SRSF2-P95H/+ cells, we have performed quantitative mass spectrometry (qMS) on K562 cells with SRSF2P95H/+ knocked into the endogenous locus (PMID 30799057) and parental K562 cells, treated with the GSK-3 inhibitor CHIR99021. We focused on the mitochondrial proteome using human proteome (UniprotKB) and mitochondrial (MitoCarta 3.0) databases for protein identification, identifying 163 mitochondrial proteins whose levels are affected by SRSF2 mutation.
HostingRepositoryPRIDE
AnnounceDate2024-06-22
AnnouncementXMLSubmission_2024-06-22_08:36:56.869.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKevin A Janssen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-22 09:30:55ID requested
12024-06-22 08:36:57announced
Publication List
Liu X, Devadiga SA, Stanley RF, Morrow RM, Janssen KA, Quesnel-Valli, รจ, res M, Pomp O, Moverley AA, Li C, Skuli N, Carroll M, Huang J, Wallace DC, Lynch KW, Abdel-Wahab O, Klein PS, A mitochondrial surveillance mechanism activated by SRSF2 mutations in hematologic malignancies. J Clin Invest, 134(12):(2024) [pubmed]
10.1172/jci175619;
Keyword List
ProteomeXchange project tag: Human Proteome Project
submitter keyword: mitophagy, chronic myelomonocytic leukemia (CMML),myelodysplasia, SRSF2, acute myeloid leukemia, splicing, nonsense mediated decay, GSK-3, PINK1
Contact List
Douglas C. Wallace
contact affiliationChildren's Hospital of Philadelphia
contact emailwallaced1@chop.edu
lab head
Kevin A Janssen
contact affiliationChildren's Hospital of Philadelphia
contact emailkxjanssen@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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