PXD043213 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeting mitophagy in SRSF2 mutant hematologic malignancies |
Description | Recurrent Glycogen synthase kinase-3 (GSK-3) phosphorylates multiple splicing factors, including SRSF2, and regulates the splicing of a broad range of mRNAs in human cells. Inhibition of GSK-3 disrupts splicing and promotes cell death in hematopoietic cells with heterozygous mutations in SRSF2 and not in cells with wild-type splicing factors. To characterize how GSK-3 inhibition alters the cellular proteome in SRSF2-P95H/+ cells, we have performed quantitative mass spectrometry (qMS) on K562 cells with SRSF2P95H/+ knocked into the endogenous locus (PMID 30799057) and parental K562 cells, treated with the GSK-3 inhibitor CHIR99021. We focused on the mitochondrial proteome using human proteome (UniprotKB) and mitochondrial (MitoCarta 3.0) databases for protein identification, identifying 163 mitochondrial proteins whose levels are affected by SRSF2 mutation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-22 |
AnnouncementXML | Submission_2024-06-22_08:36:56.869.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kevin A Janssen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-22 09:30:55 | ID requested | |
⏵ 1 | 2024-06-22 08:36:57 | announced | |
Publication List
Liu X, Devadiga SA, Stanley RF, Morrow RM, Janssen KA, Quesnel-Valli, รจ, res M, Pomp O, Moverley AA, Li C, Skuli N, Carroll M, Huang J, Wallace DC, Lynch KW, Abdel-Wahab O, Klein PS, A mitochondrial surveillance mechanism activated by SRSF2 mutations in hematologic malignancies. J Clin Invest, 134(12):(2024) [pubmed] |
10.1172/jci175619; |
Keyword List
ProteomeXchange project tag: Human Proteome Project |
submitter keyword: mitophagy, chronic myelomonocytic leukemia (CMML),myelodysplasia, SRSF2, acute myeloid leukemia, splicing, nonsense mediated decay, GSK-3, PINK1 |
Contact List
Douglas C. Wallace |
contact affiliation | Children's Hospital of Philadelphia |
contact email | wallaced1@chop.edu |
lab head | |
Kevin A Janssen |
contact affiliation | Children's Hospital of Philadelphia |
contact email | kxjanssen@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043213
- Label: PRIDE project
- Name: Targeting mitophagy in SRSF2 mutant hematologic malignancies