PXD043209

PXD043209 is an original dataset announced via ProteomeXchange.

Title	PBRM1 directs PBAF to pericentromeres and centromeres to constrain transcription and protect genome integrity
Description	The specialized structure of the centromere is critical for effective chromosome segregation, but its repetitive nature and configuration makes it vulnerable to rearrangements. Centromere fragility can drive tumorigenesis, but protective mechanisms preventing fragility are still not fully understood. To identify core functions of the tumor suppressor PBRM1, a subunit of the PBAF remodelling complex, we created a panel of isogenic cell lines. We found that compromised centromere integrity is a general feature of cells lacking PBRM1. This defect creates a dependence on the spindle assembly checkpoint, which can be therapeutically exploited. We map the SMARCA4 subunit of PBAF across centromeric and pericentromeric chromatin and find PBRM1-dependent association with specific sequences. We further find that loss of PBRM1 leads to altered transcription of pericentromere-associated genes and transposable elements. These data identify PBAF as a protector of centromere structure with profound consequences for genome integrity.
HostingRepository	PRIDE
AnnounceDate	2025-01-06
AnnouncementXML	Submission_2025-01-06_11:23:44.303.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Graeme Benstead-Hume
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-22 06:27:46	ID requested
⏵ 1	2025-01-06 11:23:44	announced

Dataset with its publication pending

submitter keyword: PBRM1, transcription, pericentromeres, centromeres, PBAF

Jyoti Choudhary
contact affiliation	Functional Proteomics, ICR
contact email	jyoti.choudhary@icr.ac.uk
lab head
Graeme Benstead-Hume
contact affiliation	ICR
contact email	ghume@icr.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD043209

PRIDE project URI

• PRIDE
  1. PXD043209
     1. Label: PRIDE project
     2. Name: PBRM1 directs PBAF to pericentromeres and centromeres to constrain transcription and protect genome integrity