PXD043207

PXD043207 is an original dataset announced via ProteomeXchange.

Title	Ribosome inactivation regulates translation elongation in neurons
Description	Cellular plasticity is a prerequisite to adapt to ever-changing stimuli. Therefore, cells constantly reshape their translatome and, in turn, their proteome. The control of translational activity has been extensively studied at the stage of translation initiation. How cells regulate polysome speed is, however, widely unknown. Here, we exploited a kinetic approach to investigate global translation kinetics in cells. We found that polysome speeds differ between different cell types including astrocytes, induced pluripotent human stem cells, human neural stem cells, and human and rat neurons. Among these cells, we observed that mature cortical neurons translate the fastest. This finding was even more striking as these cells express the elongation factor 2 (eEF2) at lowest levels compared to other cell types. We found that neurons resolve this obstacle by inactivating a fraction of their ribosomes. This process is regulated by the levels and phosphorylation of eEF2 as well as by NMDA mediated neuronal stimulation. Our data strongly suggest a novel regulation mechanism in which nerve cells inactivate ribosomes to allow for translational remodeling. This finding has important implications for developmental brain disorders that are hallmarked by altered translation.
HostingRepository	PRIDE
AnnounceDate	2024-05-10
AnnouncementXML	Submission_2024-05-10_02:11:01.184.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-22 05:00:33	ID requested
⏵ 1	2024-05-10 02:11:01	announced

10.1016/J.JBC.2024.105648;

submitter keyword: ribosome speed, astrocytes, polysome profiling, stem cells, eEF2

Dr Rico Schieweck
contact affiliation	Department of Physiological Genomics Department for Cell Biology & Anatomy Medical Faculty Biomedical Center (BMC) Ludwig-Maximilians-University, 82152 Planegg-Martinsried, Germany
contact email	r.schieweck@googlemail.com
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD043207

PRIDE project URI

• PRIDE
  1. PXD043207
     1. Label: PRIDE project
     2. Name: Ribosome inactivation regulates translation elongation in neurons