PXD043203

PXD043203 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab
Description	The Pertuzumab-binding affimer 00557_709097 was incubated either with peptides from tryptically digested Pertuzumab or with selected synthesized peptides. Affimer-peptide complexes were subjected to nano electrospray mass spectrometric ITEM analyses in order to identify the affimer’s epitope on Pertuzumab. Furthermore the affimer was incubated with intact Pertuzmab to determine apparent binding energies and dissociation constants of the complex dissociation reactions in the gas phase using ITEM-TWO.
HostingRepository	PRIDE
AnnounceDate	2024-04-09
AnnouncementXML	Submission_2024-04-08_22:20:36.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-22 04:03:28	ID requested
⏵ 1	2024-04-08 22:20:37	announced

10.3390/biom14010024;

R, ö, wer C, Olaleye OO, Bischoff R, Glocker MO, Mass Spectrometric ITEM-ONE and ITEM-TWO Analyses Confirm and Refine an Assembled Epitope of an Anti-Pertuzumab Affimer. Biomolecules, 14(1):(2023) [pubmed]

submitter keyword: Pertuzumab, ITEM, affimer,Offline nanoESI-MS

Prof. Dr. Michael O. Glocker
contact affiliation	Director, Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD043203

PRIDE project URI

• PRIDE
  1. PXD043203
     1. Label: PRIDE project
     2. Name: Mass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab