PXD043164

PXD043164 is an original dataset announced via ProteomeXchange.

Title	Plasmodium ARK2 and EB1 drives the unconventional spindle dynamics, during chromosome segregation in sexual transmission stages
Description	The Aurora family of kinases orchestrates chromosome segregation and cytokinesis during cell division, with precise spatiotemporal regulation of its catalytic activities by distinct protein scaffolds. Plasmodium spp., the causative agents of malaria, are unicellular eukaryotes with three unique and highly divergent aurora-related kinases (ARK1-3) that are essential for asexual cellular proliferation, but lack most canonical scaffolds/activators. Here we investigate the role of ARK2 during sexual proliferation of the rodent malaria Plasmodium berghei, using a combination of super-resolution microscopy, mass spectrometry, and live-cell fluorescence imaging. We find that ARK2 is primarily located at spindle apparatus microtubules in the vicinity of kinetochores during both mitosis and meiosis. Interactomic and co-localisation studies reveal several putative ARK2-associated interactors including the microtubule plus end-binding protein EB1, together with MISFIT and Myosin-K, but no conserved eukaryotic scaffold proteins. Gene function studies indicate that ARK2 and EB1 are complementary in driving endomitotic division and thereby parasite transmission through the mosquito. This discovery underlines the flexibility of molecular networks to rewire and drive unconventional mechanisms of chromosome segregation in the malaria parasite.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:38.222.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043164
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-21 05:26:14	ID requested
1	2023-08-15 04:20:43	announced
⏵ 2	2023-11-14 09:05:01	announced	2023-11-14: Updated project metadata.

10.6019/PXD043164;

Zeeshan M, Rea E, Abel S, Vuku, š, i, ć K, Markus R, Brady D, Eze A, Rashpa R, Balestra AC, Bottrill AR, Brochet M, Guttery DS, Toli, ć IM, Holder AA, Le Roch KG, Tromer EC, Tewari R, Plasmodium ARK2-EB1 axis drives the unconventional spindle dynamics, scaffold formation and chromosome segregation of sexual transmission stages. Res Sq, ():(2023) [pubmed]

submitter keyword: Plasmodium, Warwick_RTP, ARK2, EB1

Professor Rita Tewari
contact affiliation	School of Life Sciences, University of Nottingham, Nottingham, UK.
contact email	rita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043164
     1. Label: PRIDE project
     2. Name: Plasmodium ARK2 and EB1 drives the unconventional spindle dynamics, during chromosome segregation in sexual transmission stages