Eimeria tenella, one of the pathogens of poultry coccidiosis, is an absolute intracellular parasite belong to phylum Apicomplexa. The parasite is highly host-specific and presents a monoxenous life cycle with multiple developmental stages. Each stage of E. tenella has different morphology, virulence, gene and protein expressions which may change through gene regulatory and post-translational modifications (PTMs) machineries. Dissecting the regulatory mechanism for E. tenella stage conversion is attractive and essential to understand parasite-host interaction. In the current study, the ubiquitome data of five developmental stages of E. tenella (unsporulated oocyst, onset of sporulation i.e., seven hours into sporulation of oocyst, sporulated oocyst, sporozoite and second-generation merozoite) were deciphered, and ubiquitomic comparison of the 5 stages were revealed. Substantially, ubiquitin conjugates were detected dynamically changed during several stages, and the protein degradation controlled by ubiquitination machinery could be the basis why some proteins accumulate and others do not in different life stages of E. tenella. In brief, we revealed the presence of ubiquitination in E. tenella and our results lay the foundation for a deeper understanding of ubiquitination and its significance in the life cycle of the avian parasite.