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PXD043152

PXD043152 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEdible mycelium bioengineered for enhanced nutritional value and sensory appeal using a new synthetic biology toolkit
DescriptionGenetic engineering of filamentous fungi has promise for accelerating the transition to a more sustainable food system and enhancing the nutritional value, sensory appeal, and scalability of microbial foods. However, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we developed a synthetic biology toolkit for Aspergillus oryzae, an edible fungus traditionally used in fermented foods and currently used in protein production and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for genome integration, neutral loci, and new promoters. We use these tools to enhance the elevate levels of the nutraceutical ergothioneine and intracellular heme in the edible biomass. The biomass overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of genetic approaches to enhance fungal meat alternatives and provide useful engineering tools for diverse applications in fungal food production and beyond.
HostingRepositoryPRIDE
AnnounceDate2024-03-22
AnnouncementXMLSubmission_2024-03-22_11:49:24.762.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristopher Petzold
SpeciesList scientific name: Aspergillus oryzae; NCBI TaxID: 5062;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-20 20:50:03ID requested
12024-03-22 11:49:25announced
Publication List
Maini Rekdal V, van der Luijt CRB, Chen Y, Kakumanu R, Baidoo EEK, Petzold CJ, Cruz-Morales P, Keasling JD, Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. Nat Commun, 15(1):2099(2024) [pubmed]
10.1038/s41467-024-46314-8;
Keyword List
submitter keyword: Bioengineering, nutrition, synthetic biology, Edible mycelium
Contact List
Christopher J. Petzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
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Dataset FTP location
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PRIDE project URI
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