PXD043152 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a new synthetic biology toolkit |
Description | Genetic engineering of filamentous fungi has promise for accelerating the transition to a more sustainable food system and enhancing the nutritional value, sensory appeal, and scalability of microbial foods. However, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we developed a synthetic biology toolkit for Aspergillus oryzae, an edible fungus traditionally used in fermented foods and currently used in protein production and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for genome integration, neutral loci, and new promoters. We use these tools to enhance the elevate levels of the nutraceutical ergothioneine and intracellular heme in the edible biomass. The biomass overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of genetic approaches to enhance fungal meat alternatives and provide useful engineering tools for diverse applications in fungal food production and beyond. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-22 |
AnnouncementXML | Submission_2024-03-22_11:49:24.762.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christopher Petzold |
SpeciesList | scientific name: Aspergillus oryzae; NCBI TaxID: 5062; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-20 20:50:03 | ID requested | |
⏵ 1 | 2024-03-22 11:49:25 | announced | |
Publication List
Maini Rekdal V, van der Luijt CRB, Chen Y, Kakumanu R, Baidoo EEK, Petzold CJ, Cruz-Morales P, Keasling JD, Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit. Nat Commun, 15(1):2099(2024) [pubmed] |
10.1038/s41467-024-46314-8; |
Keyword List
submitter keyword: Bioengineering, nutrition, synthetic biology, Edible mycelium |
Contact List
Christopher J. Petzold |
contact affiliation | Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 |
contact email | cjpetzold@lbl.gov |
lab head | |
Christopher Petzold |
contact affiliation | Lawrence Berkeley National Laboratory |
contact email | cjpetzold@lbl.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043152
- Label: PRIDE project
- Name: Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a new synthetic biology toolkit