PXD043149
PXD043149 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Autolysosomal SNX16 regulates autophagosomal components recycling |
| Description | In autophagy, autophagosomes fuse with lysosomes to form autolysosomes. Contents enclosed within autophagosomes were degraded, while autophagosome outer membrane components are recycled from autolysosomes by the recycler complex via autophagosomal components recycling (ACR). However, the detailed mechanism for this recycling process remains poorly understood. By overlapping the recycler complex subunit SNX4 interacting proteins and proteins biotinylated by the recycling cargo APEX-STX17, we identified SNX16 as a key protein in ACR. Further analysis showed that SNX16 localizes to autolysosomes and is required for ACR. SNX16 interacts with the recycler complex and functions in ACR by regulating the recycler complex formation, facilitating cargo recognition and the connection between cargoes-SNX4-SNX5 and dynein-dynactin complexes. In addition, SNX16-cargoes interactions are regulated by the activity of two ACR-related small GTPases, Rab32 and Rab38. Taken together, our research unveiled a crucial role of SNX16 in ACR and provided insights into its function on autolysosomes, aside from its previously known functions on endosomes. |
| HostingRepository | iProX |
| AnnounceDate | 2023-06-21 |
| AnnouncementXML | Submission_2023-06-20_19:31:15.115.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shuaixin Gao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-20 19:30:51 | ID requested | |
| ⏵ 1 | 2023-06-20 19:31:15 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Autolysosoma, SNX16, SNX17 |
Contact List
| Yueguang Rong | |
|---|---|
| contact affiliation | Cell Architecture Research Center, Huazhong University of Science and Technology |
| contact email | rongyueguang@hust.edu.cn |
| lab head | |
| Shuaixin Gao | |
| contact affiliation | Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University |
| contact email | gaoshuaixin@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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