PXD043146

PXD043146 is an original dataset announced via ProteomeXchange.

Title	Exosomal proteomic profiling revealed children type 1 diabete pathogenesis and potential therapeutic targets
Description	Abstract Introduction: Type 1 diabetes (T1D) is a serious autoimmune disease with high morbidity and mortality. Early diagnosis and treatment remain unsatisfactory. Circulating exosomes containing T1D biomarkers have gained interest, but progress is limited. Objectives: This study investigates molecular dynamics of plasma exosomes in pediatric T1D patients and reveals potential mechanisms associated with T1D development. Methods: Liquid chromatography-tandem mass spectrometry with TMT6 labeling quantified exosomal protein expression profiles in 12 healthy and 24 pediatric T1D patients, stratified by age (≤6 years and >6 years) and glycated hemoglobin (HbA1c) levels (>7% or ≤7%). Integrative bioinformatics analyses were conducted, and Western Blot (WB) validated findings. Results: We identified 1035 proteins, revealing 548 and 589 differentially expressed proteins (fold change >1.3) in T1D patients aged ≤6 and >6 years, respectively. When HbA1c was below 7% (received insulin therapy for at least three months), most of the altered protein levels in both age groups resembled healthy controls. Bioinformatics analysis indicated exosome components related to immune functions, hemostasis, cellular stress response, and extracellular matrix organization. WB confirmed alterations in RAB40A, COL6A5, SEMA6D, and TTR proteins. Conclusion: Our study characterizes plasma exosome protein dynamics in pediatric T1D patients across age stages, providing valuable insights into molecular mechanisms and identifying potential therapeutic targets for T1D management.
HostingRepository	iProX
AnnounceDate	2023-06-20
AnnouncementXML	Submission_2023-11-14_23:20:48.949.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baoling Bai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	TMT6plex reporter fragment
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-20 19:16:41	ID requested
1	2023-06-20 19:18:47	announced
⏵ 2	2023-11-14 23:20:49	announced	2023-11-15: Update publication information.

Bai B, Gao K, Zhang K, Liu L, Chen X, Zhang Q, Pathological mechanisms of type 1 diabetes in children: investigation of the exosomal protein expression profile. Front Endocrinol (Lausanne), 14():1271929(2023) [pubmed]

submitter keyword: Type 1 diabetes, Exosome, Proteomics,TMT6

Qin Zhang
contact affiliation	Capital Institute of Pediatrics
contact email	maureenzq@hotmail.com
lab head
Baoling Bai
contact affiliation	Capital Institute of Pediatrics
contact email	baoxiang8802@126.com
dataset submitter

iProX dataset URI