PXD043135

PXD043135 is an original dataset announced via ProteomeXchange.

Title	STPP-UP: A alternative method for drug target identification using protein thermal stability
Description	Thermal proteome profiling (TPP) has significantly advanced the field of drug discovery by facilitating proteome-wide identification of drug targets and off-targets. However, TPP has not been widely applied for high-throughput drug screenings, since the method is labor intensive and requires a lot of measurement time on a mass spectrometer. Here, we present Single-tube TPP with Uniform Progression (STPP-UP), which significantly reduces both the amount of required input material and measurement time, while retaining the ability to identify drug targets for compounds of interest.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:38.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dick Zijlmans
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-20 03:55:11	ID requested
1	2023-10-24 05:43:58	announced
⏵ 2	2023-11-14 08:25:38	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: protein stability, drug discovery, thermal proteomics,STPP-UP

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands. Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
contact email	michiel.vermeulen@ru.nl
lab head
Dick Zijlmans
contact affiliation	Radboud Universiteit Nijmegen
contact email	d.zijlmans@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043135

PRIDE project URI

• PRIDE
  1. PXD043135
     1. Label: PRIDE project
     2. Name: STPP-UP: A alternative method for drug target identification using protein thermal stability